The optimal therapeutic timing and mechanism of puerarin treatment of spinal cord ischemia-reperfusion injury in rats
The optimal therapeutic timing and mechanism of puerarin treatment of spinal cord ischemia-reperfusion injury in rats
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DOI：
10.1016/j.jep.2011.01.055
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发表时间：
2011-04-12
影响因子：
5.4
通讯作者：
Ji, Xiang-Lu
中科院分区：
文献类型：
--
作者：
Tian, Feng;Xu, Li-Hui;Ji, Xiang-Lu
研究目的：本研究旨在探讨葛根素治疗脊髓缺血再灌注损伤的最佳治疗时机及机制。材料与方法：雄性SD大鼠脊髓缺血再灌注损伤，于损伤后1、2、4、6 h腹腔注射葛根素50 mg/kg。再灌注开始后48 h测定运动功能。硫氧还蛋白的表达和细胞凋亡indicsdetermined.Results：运动功能的改善，在1，2，和4小时，证明在动物与葛根素治疗。缺血再灌注损伤导致硫氧还蛋白表达减少，而葛根素给药升高硫氧还蛋白-1/硫氧还蛋白-2 mRNA的表达。细胞凋亡指数显着降低葛根素administration.Conclusions：我们的结论是，在4小时内脊髓缺血再灌注损伤的葛根素管理减少缺血再灌注损伤，葛根素的神经保护作用涉及的硫氧还蛋白的转录增加和细胞凋亡的减少。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Aim of the study: The purpose of this study was to explore the optimal therapeutic timing and mechanism of puerarin treatment of spinal cord ischemia-reperfusion injury.Materials and methods: The spinal ischemia-reperfusion injury was conducted in male Sprague-Dawley rats, and 50 mg/kg of puerarin was injected intraperitoneally at 1, 2, 4 and 6 h after the injury. Motor function was measured 48 h after reperfusion started. Thioredoxin expression and apoptosis indices were determined.Results: Improvement of motor function at 1, 2, and 4 h was demonstrated in the animals with puerarin treatment. Ischernia-reperfusion injury resulted in a decrease in the expression of thioredoxin, while puerarin administration elevated the expression of thioredoxin-1 /thioredoxin-2 mRNA. Apoptosis indices were significantly reduced by puerarin administration.Conclusions: We conclude that administration of puerarin within 4 h of spinal ischemia-reperfusion injury reduces ischemic reperfusion damage, and that the neuroprotective effect of puerarin involves an increase in the transcription of thioredoxin and a reduction of apoptosis. (C) 2011 Elsevier Ireland Ltd. All rights reserved.