KINKED DNA IN CRYSTALLINE COMPLEX WITH ECORI ENDONUCLEASE
KINKED DNA IN CRYSTALLINE COMPLEX WITH ECORI ENDONUCLEASE
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DOI：
10.1038/309327a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
ROSENBERG, JM
中科院分区：
文献类型：
--
作者：
FREDERICK, CA;GRABLE, J;ROSENBERG, JM
3.ANG。 EcoRI 核酸内切酶和寡核苷酸 TCGCGAATTCGCG 之间的共晶识别复合物的电子密度图表明，酶和 DNA 大沟之间紧密、互补的界面是序列特异性的主要决定因素。 DNA 包含扭转扭结和其他与 B 构象的偏离，这些偏离使 DNA 解旋，从而加宽识别位点的主沟。
The 3 .ANG. electron density map of a co-crystalline recognition complex between EcoRI endonuclease and the oligonucleotide TCGCGAATTCGCG reveals that a tight, complementary interface between the enzyme and the major groove of the DNA is the major determinant of sequence specificity. The DNA contains a torsional kink and other departures from the B conformation which unwind the DNA and thereby widen the major groove in the recognition site.