Analysis of selection effect based on kappa casein gene on milk yield production of Iranian Sarabi cattle breed using stochastic simulation.
Analysis of selection effect based on kappa casein gene on milk yield production of Iranian Sarabi cattle breed using stochastic simulation.
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DOI：
10.3923/pjbs.2007.941.945
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发表时间：
2007-03
期刊：
影响因子：
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通讯作者：
S. Zakizadeh;A. Nejati-Javaremi;M. Reissmann;G. Rahimi;A. J. Bakhshi
中科院分区：
文献类型：
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作者：
S. Zakizadeh;A. Nejati-Javaremi;M. Reissmann;G. Rahimi;A. J. Bakhshi
采用 PCR-RFLP 对伊朗西北部 87 头萨拉比本地牛进行了 kappa 酪蛋白基因 A 和 B 等位基因的基因分型。外显子 4 和内含子 4 的 350 bp 长度被扩增并用 HinfI 核酸内切酶消化。将样品加载到琼脂糖凝胶 (2%) 上并在紫外光下进行基因分型。理想的 B 等位基因的等位基因频率为 0.57。使用随机模拟为 15 个重叠世代的 4950 名女性和 50 名男性群体生成产奶量性状。群体参数包括平均产奶量和表型偏差分别为 1100 公斤和 436 公斤；遗传力为0.27。 Kappa酪蛋白基因的加性效应和显性效应分别被认为是187.63和50.37 kg。考虑了两种方法来根据其母体的第一个表型记录（PAS）或每个雄性的分子信息（GAS）来选择雄性。雌性总是在其第一个表型记录时被选择。虽然两种方法在第5代后多基因和主基因的遗传反应存在显着差异，但多基因和主基因反应的总和没有显着差异。经过15代选择后，两种方法的近交系数无显着差异。在萨拉比本地品种中，基于单一主基因的雄性选择计划与基于母体记录的传统选择相比没有优势。
PCR-RFLP was used to genotype 87 Sarabi native cattle of north-western Iran for A and B alleles of kappa casein gene. A 350 bp length of exon 4 and intron 4 was amplified and digested with HinfI endonuclease. Samples were loaded on agarose gel (2%) and genotyped under UV light. Allele frequency of desirable B allele was 0.57. Stochastic simulation was used to generate milk yield trait for a population of 4950 females and 50 males for 15 overlapping generations. Population parameters included 1100 and 436 kg for average milk yield and phenotypic deviation, respectively; with heritability of 0.27. Additive and dominance effects of Kappa Casein gene were considered as 187.63 and 50.37 kg, respectively. Two methods were considered for selection of males based on the first phenotypic record of their dams (PAS) or molecular information of each male, individually (GAS). Females were always selected on their first phenotypic record. Although, there was a significant difference between polygenic and major gene genetic response between two methods after the 5th generations, but there was no significant difference for the sum of polygenic and major gene response. After 15 generations of selection there was no significant difference between inbreeding coefficient under two methods. Selection plan for males based on one single major gene had no advantage over the conventional selection based on dam record in native Sarabi breed.