Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death
Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death
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DOI：
10.1016/j.bbrc.2008.11.156
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发表时间：
2009-02-06
影响因子：
3.1
通讯作者：
Nam, Kyoung Hee
中科院分区：
文献类型：
--
作者：
Ryu, Hee Young;Kim, Sun Young;Nam, Kyoung Hee
谷胱甘肽-S-转移酶是参与细胞解毒的必需蛋白质。GST的表达已被广泛研究在各种环境应激，包括外源性物质。在这里，我们已经分离出AtGST 10，其中一个phi类AtGST的基础上，它与BAK 1在酵母双杂交筛选的相互作用。BAK 1是LRR-RLK。在油菜素类固醇信号传导和植物防御反应中起作用。我们发现AtGSTF 10通过其N端结构域与BAK 1结合。AtCSTF 10在植物组织中广泛表达，与其他GST不同，AtGSTF 10的内源转录水平不受植物生长调节剂或非生物胁迫诱导，除了干旱。AtGSTF 10的过表达赋予转基因植物更高的耐盐性和受干扰的氧化还原状态。通过RNA干扰下调AtGSTF 10的表达导致转化体对非生物胁迫的耐受性降低和加速衰老，表明AtGSTF 10参与拟南芥的胁迫耐受性和BAK 1介导的自发细胞死亡信号通路。(c)2008年爱思唯尔公司All rights reserved.
Glutathione-S-transferases are essential proteins involved in cellular detoxification. The expression of GSTs has been studied extensively under various environmental stressors including xenobiotics. Here, we have isolated AtGST10, one of the phi classes of AtGSTs on the basis of its interaction with BAK1 in a yeast two-hybrid screen. BAK1 is an LRR-RLK. acting in both brassinosteroid signaling and plant defense responses. We found that AtGSTF10 binds to BAK1 through its N-terminal domain. AtCSTF10 is expressed ubiquitously in plant tissues, and the endogenous transcript level of AtGSTF10 was not induced by plant growth regulators or abiotic stressors, except drought, unlike other GSTs. Overexpression of AtGSTF10 conferred higher tolerance to salt and disturbed redox status of transgenic plants. The down-regulation of AtGSTF10 produced by RNA interference caused reduced tolerance to abiotic stress and an accelerated senescence of transformants, indicating that AtGSTF10 is involved in stress tolerance and the BAK1-mediated spontaneous cell death signaling pathway in Arabidopsis. (c) 2008 Elsevier Inc. All rights reserved.