ABNORMALITY OF WATER BARRIER FUNCTION IN PSORIASIS - ROLE OF CERAMIDE FRACTIONS
ABNORMALITY OF WATER BARRIER FUNCTION IN PSORIASIS - ROLE OF CERAMIDE FRACTIONS
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DOI：
10.1001/archderm.130.4.452
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发表时间：
1994-04-01
影响因子：
--
通讯作者：
CAPUTO, R
中科院分区：
文献类型：
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作者：
MOTTA, S;MONTI, M;CAPUTO, R
背景与设计：银屑病中皮质层的形成紊乱，据报道水通量增加。我们研究了三种不同形式的银屑病：在未受累皮肤和银屑病斑块上测量经表皮水分损失;提取斑块中的脂质;比较鳞片与正常角质层中神经酰胺的分布。此外，脂质生化结果与相同病变的经皮水分丢失率进行了比较。为了评估神经酰胺分布的潜在变化，通过Bligh-Dyer方法提取银屑病鳞屑和正常角质层中的脂质，在高效薄层色谱板上分离;并通过计算机密度测定法定量。水通量测量为transepidermal水分流失使用appropriometer;未涉及和涉及银屑病皮肤和年龄匹配的对照皮肤之间的结果进行了统计evaluated.Results：在与正常角质层相比，银屑病斑块神经酰胺表现出不同的分布，特别是，神经酰胺1显着下降。银屑病斑块与对照皮肤之间的银屑病涉及与未涉及的skin.Conclusion：我们的研究结果表明，在银屑病的神经酰胺分布的改变，可以具体链接到角质形成细胞分化的缺陷，皮肤屏障功能的缺陷可能是由于很大程度上或部分神经酰胺1减少的经表皮水分流失值增加。
Background and Design: In psoriasis the formation of the cornified layer is deranged and water flux is reportedly increased. We investigated three different forms of psoriasis: transepidermal water loss was measured on uninvolved skin and psoriatic plaques; lipids from plaques were extracted; and ceramide distribution in scale vs normal stratum corneum was compared. Moreover, the lipid biochemical results were compared with transepidermal water loss rates in the same lesions. To assess potential alteration in ceramide distribution, lipids from both psoriatic scale and normal stratum corneum were extracted by the Bligh-Dyer method, separated on high performance thin layer chromatography plates; and quantified by computerized densitometry. Water flux was measured as transepidermal water loss using an evaporimeter; results between uninvolved and involved psoriatic skin and age-matched control skin were statistically evaluated.Results: In comparison with normal stratum corneum, psoriatic plaque ceramides showed a different distribution; in particular, ceramide 1 was significantly decreased. The increased transepidermal water loss values of psoriatic plaques vs control skin and between psoriatic involved vs uninvolved skin were significant.Conclusion: Our findings indicate that in psoriasis the altered ceramide distribution can be linked specifically to the defect in keratinocyte differentiation; the defect in skin barrier function may be attributed largely or in part to ceramide 1 reduction.