Imaging targeted-agent binding in vivo with two probes
Imaging targeted-agent binding in vivo with two probes
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DOI：
10.1117/1.3449109
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发表时间：
2010-05-01
影响因子：
3.5
通讯作者：
Hasan, Tayyaba
中科院分区：
文献类型：
--
作者：
Pogue, Brian W.;Samkoe, Kimberley S.;Hasan, Tayyaba
通过双探针注射靶向和非靶向荧光染料，演示了一种对体内靶向剂结合率进行定量成像的方法。创建的结合率常数图像显示，与正常胰腺 (3.4 x 10(-5) s(-1)) 相比，原位异种移植胰腺肿瘤 (2.3 x 10(-5) s(-1)) 中表皮生长因子的摄取低于预期。这种方法允许对体内肿瘤受体靶向进行无创评估，以确定预期的对比度、空间定位和治疗剂递送的功效。 (C) 2010 年光电仪器工程师协会。 [DOI：10.1117/1.3449109]
An approach to quantitatively image targeted-agent binding rate in vivo is demonstrated with dual-probe injection of both targeted and nontargeted fluorescent dyes. Images of a binding rate constant are created that reveal lower than expected uptake of epidermal growth factor in an orthotopic xenograft pancreas tumor (2.3 x 10(-5) s(-1)), as compared to the normal pancreas (3.4 x 10(-5) s(-1)). This approach allows noninvasive assessment of tumor receptor targeting in vivo to determine the expected contrast, spatial localization, and efficacy in therapeutic agent delivery. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3449109]