COMPARISONS OF TRITIATED THYMIDINE, TRITIATED WATER, AND COBALT-60 GAMMA RAYS IN INDUCING CHROMOSOMAL ABERRATIONS.
COMPARISONS OF TRITIATED THYMIDINE, TRITIATED WATER, AND COBALT-60 GAMMA RAYS IN INDUCING CHROMOSOMAL ABERRATIONS.
复制标题
氚化胸苷、氚化水和钴 60 伽马射线诱导染色体畸变的比较。
作者：
W. Dewey;R. Humphrey;B. Jones
有报道称，氚标记胸苷(H3TdR)掺入脱氧核糖核酸(DNA)可抑制组织培养细胞和腹水癌细胞的生存和生长(1-4)；对鸡胚产生细胞学效应(5)；导致大鼠肝脏多倍体细胞增加(6)；破坏精原细胞(4、7、8)；引起植物(9-11)、白细胞(12)和再生大鼠肝细胞(13)的染色体畸变；诱发小鼠肿瘤(14)；对小鼠(15)和细菌(16-18)产生致命性和突变。除了细菌工作，这些研究中的大多数都不是用核内掺入量的术语来量化的，既不是用细胞中氚分解的数量来衡量，也不是用引起所观察到的效应所需的辐射剂量来衡量。本研究的目的是(1)确定可见染色体畸变数和DNA中氚解体数之间的关系，以及(2)比较产生一定量染色体损伤所需的H3TdR辐射剂量与产生相同量损伤所需的慢性辐射剂量和辐射剂量。
Reports have indicated that ,-radiation from tritium-labeled thymidine (H3TdR) incorporated into deoxyribonucleic acid (DNA) inhibits the survival and growth of tissue culture cells and ascites tumor cells (1-4); produces cytological effects in chick embryos (5); causes an increase in polyploid cells in the rat liver (6); damages spermatogonia (4, 7, 8); causes chromosome aberrations in plants (9-11), leukocytes (12), and regenerating rat liver cells (13); induces tumors in mice (14); and produces both lethality and mutations in mice (15) and bacteria (16-18). Except for the bacterial work, most of these studies are not quantitative in terlms of the amount of intranuclear incorporation measured either as the number of tritium disintegrations in the cell or as the dose in rads required to cause the effects observed. The object of the present study is (1) to determine the relationship between the number of visible chromosomal aberrations and the number of tritium disintegrations in the DNA, and (2) to compare the radiation dose delivered from H3TdR required to produce a certain amount of chromosomal damage with the radiation dose from chronic fAand y-radiation required to produce the same amount of damage.