A mass spectrometry method for the determination of the species of origin of gelatine in foods and pharmaceutical products

A mass spectrometry method for the determination of the species of origin of gelatine in foods and pharmaceutical products
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DOI:
10.1016/j.foodchem.2015.05.054
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发表时间:
2016-01-01
期刊:
影响因子:
8.8
通讯作者:
Collins, M. J.
Collins, M. J.
中科院分区:
农林科学1区
文献类型:
--
作者:
Grundy, H. H.;Reece, P.;Collins, M. J.

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明胶是一个组成部分的范围广泛的食物。它是肉类工业的副产品,由骨头和兽皮制成,主要来自牛和猪。准确的食品标签使消费者能够对他们购买的食品做出明智的决定。由于标签目前在很大程度上依赖于涉及文件线索的尽职调查,因此在明胶的物种来源方面为消费行业开发可靠的测试方法可能是有益的。我们提出了一种用肽质谱法确定明胶种属来源的方法。并与ELISA和PCR技术进行了评价性比较。发现商业明胶含有未申报的物质。此外,在商业注射基质中观察到未申报的牛肽。因此,这种分析方法可以支持食品工业确定食品中明胶的物种真实性。皇冠版权所有(C)2015由爱思唯尔有限公司出版。保留所有权利。
Gelatine is a component of a wide range of foods. It is manufactured as a by-product of the meat industry from bone and hide, mainly from bovine and porcine sources. Accurate food labelling enables consumers to make informed decisions about the food they buy. Since labelling currently relies heavily on due diligence involving a paper trail, there could be benefits in developing a reliable test method for the consumer industries in terms of the species origin of gelatine. We present a method to determine the species origin of gelatines by peptide mass spectrometry methods. An evaluative comparison is also made with ELISA and PCR technologies. Commercial gelatines were found to contain undeclared species. Furthermore, undeclared bovine peptides were observed in commercial injection matrices. This analytical method could therefore support the food industry in terms of determining the species authenticity of gelatine in foods. Crown Copyright (C) 2015 Published by Elsevier Ltd. All rights reserved.