Use of SDS to extract sorghum and maize proteins for free zone capillary electrophoresis (FZCE) analysis
Use of SDS to extract sorghum and maize proteins for free zone capillary electrophoresis (FZCE) analysis
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DOI：
10.1094/cchem.2001.78.1.84
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发表时间：
2001
期刊：
影响因子：
2.4
通讯作者：
S. Bean;C. Hicks;M. Tuinstra;G. Lookhart
中科院分区：
文献类型：
--
作者：
S. Bean;C. Hicks;M. Tuinstra;G. Lookhart
ABSTRACT Two different extraction methods for extracting sorghum (Sorghum bicolor L. Moench.) storage proteins for free zone capillary electrophoresis (FZCE) analysis were compared. A traditional solvent based on 60% t-butanol was compared with a pH 10 borate buffer containing the anionic detergent SDS followed by precipitation of nonkafirins using 60% t-butanol. FZCE analysis of both types of extracts showed identical patterns, despite the fact that the SDS should have given all proteins equal charge-to-mass ratios. This methodology was also successfully applied to maize proteins. The use of t-butanol to precipitate nonkafirins, combined with electrophoresis at low pH, is thought to have removed the SDS from the storage proteins. The SDS extraction procedure produced more stable extracts for FZCE analysis. These extracts could also be used directly for SDS capillary electrophoresis (SDS-CE) separations. Kafirins from 15 genotypes were extracted with this procedure and analyzed by FZCE and SDS-CE. Resolut...
