A microchannel immunoassay chip with ferrofluid actuation to enhance the biochemical reaction
A microchannel immunoassay chip with ferrofluid actuation to enhance the biochemical reaction
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DOI：
10.1016/j.snb.2013.03.078
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发表时间：
2013-06
影响因子：
8.4
通讯作者：
Yaw-Jen Chang;Chih-Yu Hu;Chu-Hsuan Lin
中科院分区：
文献类型：
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作者：
Yaw-Jen Chang;Chih-Yu Hu;Chu-Hsuan Lin
本文介绍了一种由硝化棉玻片和微通道膜组成的新型微通道免疫分析芯片。该微通道膜具有一个圆形通道和四个反应区，并使用PDMS制备。分析试剂，如蛋白质，可以固定在硝化棉玻片上的反应区，待分析的样品由磁流体操纵，通过反应区循环进行免疫学测试。圆形通道中的磁流体形成塞由固定在步进电机上的外部NdFeB永磁体驱动。循环流体驱动的目的是产生足够的搅动，以增加分子碰撞的机会，从而提高反应速度。免疫分析后，可剥离PDMS微通道膜进行荧光扫描。在这项研究中，四种不同浓度的生物素分别固定在每个区域。链霉亲和素-Cy5结合物是由磁流体驱动的。实验结果表明，适当的流体驱动有助于增强免疫分析反应。可以缩短孵化时间，提供高通量的实验。此外，缓慢的流体驱动不能产生足够的搅拌来提高反应速度，而快速的流体驱动会对免疫分析产生负面影响，因为固定化的生物素可能会从芯片表面移除。
This paper presents a novel microchannel immunoassay chip comprised of a nitrocellulose slide and a microchannel film. The microchannel film has a circular channel and four reaction zones and is fabricated using PDMS. An analytical reagent, such as protein, can be immobilized at the reaction zones on a nitrocellulose slide, and the sample to be analyzed is manipulated by ferrofluid to circulate through the reaction zones for immunological tests. The ferrofluid forming plugs in the circular channel was actuated by an external NdFeB permanent magnet affixed to a stepper motor. The purpose of circulative fluid actuation is to produce enough agitation to increase the collision opportunities of molecules and, hence, the reaction rate. After immunoassay, the PDMS microchannel film can be peeled off for fluorescence scanning. In this study, four different concentrations of biotin were immobilized at each zone, respectively. Streptavidin–Cy5 conjugate was driven by ferrofluid. The experimental results showed that adequate fluid actuation was contributive to enhance the immunoassay reactions. The incubation time can be reduced, providing high throughput experiments. Moreover, slow fluid actuation cannot produce enough agitation to increase the reaction rate, while rapid fluid actuation has a negative effect on immunoassay, as the immobilized biotins may be removed from the chip surface.