Innovative robust basophil activation test using a novel gating strategy reliably diagnosing allergy with full automation
Innovative robust basophil activation test using a novel gating strategy reliably diagnosing allergy with full automation
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DOI：
10.1111/all.14900
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发表时间：
2021-05-28
期刊：
影响因子：
12.4
通讯作者：
Jappe, Uta
中科院分区：
文献类型：
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作者：
Behrends, Jochen;Schwager, Christian;Jappe, Uta
过敏是欧洲最常见的慢性疾病之一。因此，对真正检测过敏的特异性和敏感性诊断测试的需求增加了。本研究旨在建立一种高特异性、高通量和自动化的嗜碱性粒细胞激活试验（BAT），以最大概率证明过敏的存在。方法对1104份标本进行BAT分析；建立了一种包含三种抗体（Fc epsilon RI alpha, CD203c, CD63）的新型门控策略，并与我们发表的方案（12种抗体）进行了比较。基于新的门控策略，优化了存储条件、自动测量和使用R（1389个样品中的1376个）进行分析，建立了高通量的BAT。结果新型三种抗体（Fc epsilon RI alpha, CD203c, CD63）与12种抗体门控策略之间的敏感性和特异性均无差异，自动化和人工分析样品之间的敏感性和特异性均无差异（节省高达90%的劳动时间）。献血后测定嗜碱性粒细胞活化的时间已大大延长（全血储存）
Background Allergy is one of the most common chronic diseases in Europe. Therefore, an increased need for specific and sensitive diagnostic tests that truly detect allergy exists. This study aimed at establishing a highly specific high-throughput and automated basophil activation test (BAT) that proves the existence of an allergy with utmost probability.Methods BAT from 1104 samples was analyzed; a novel gating strategy with three antibodies (Fc epsilon RI alpha, CD203c, CD63) was established and compared with our published protocol (12 antibodies). Based on the novel gating strategy, storage conditions, automated measurement, and analyses using R (1376 samples out of 1389) were optimized to set up a high-throughput BAT.Results No differences in sensitivity and specificity were found between the novel three antibody (Fc epsilon RI alpha, CD203c, CD63) and the 12 antibody gating strategy or between automated and manually analyzed samples (saving up to 90% of labor time). The time frame for basophil activation measurement after blood donation has been extended considerably (whole blood storage