Mutational analysis of BRAF and K-ras in gastric cancers:: absence of BRAF mutations in gastric cancers
Mutational analysis of BRAF and K-ras in gastric cancers:: absence of BRAF mutations in gastric cancers
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DOI：
10.1007/s00439-003-1027-0
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发表时间：
2003-12-01
期刊：
影响因子：
5.3
通讯作者：
Park, JG
中科院分区：
文献类型：
--
作者：
Kim, IJ;Park, JH;Park, JG
最近，在多种人类癌症中发现了BRAF突变。有趣的是，最常见的BRAF突变（V599E）尚未在K-ras突变的肿瘤中发现。尽管大多数人类癌症类型已经筛查了BRAF突变，但尚未对胃癌进行详细的研究。因此，我们决定研究BRAF突变在胃癌中的发生率，以及BRAF和K-ras突变在胃癌中的关系。在66例胃癌和16株胃癌细胞系中发现3个非致病性BRAF多态性和7个K-ras错义突变。虽然只有9%的胃癌组有K-ras突变，但BRAF突变的发生率并不高。因此，存在于多种其他人类癌症中的BRAF突变似乎与胃癌的发展无关。
Recently, BRAF mutations were found in a variety of human cancers. Interestingly, the most common of BRAF mutation (V599E) has not been identified in tumors with K-ras mutations. Whereas the majority of human cancer types has been screened for BRAF mutations, no detailed studies on gastric cancers have been investigated. Thus, we decided to investigate the incidence of BRAF mutations in gastric cancers, and the relationship between BRAF and K-ras mutations in such cancers. Three non-pathogenic BRAF polymorphisms and seven K-ras missense mutations were found in 66 gastric cancers and 16 gastric cancer cell lines. Although only 9% of our gastric cancer panels had K-ras mutations, the incidence of BRAF mutations was not high. Thus, BRAF mutations, which are present in a variety of other human cancers, do not seem to be involved in gastric cancer development.