Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells
Splenic and peritoneal B-1 cells differ in terms of transcriptional and proliferative features that separate peritoneal B-1 from splenic B-2 cells
复制标题
DOI：
10.1006/cimm.2001.1860
复制
发表时间：
2001-10-10
影响因子：
4.3
通讯作者：
Rothstein, TL
中科院分区：
文献类型：
--
作者：
Fischer, GM;Solt, LA;Rothstein, TL
B-1细胞构成具有特征性表型和功能特征的独特B细胞亚群。B-1细胞在腹膜淋巴细胞中高度表达;大量B-1细胞也位于脾组织中。在此鉴定了分离腹膜B-1和脾B-2细胞的转录因子和基因表达的许多差异，然后测试从免疫球蛋白转基因小鼠获得的脾B-1细胞的这些参数。脾B-1细胞与脾B-2细胞相似，而与腹膜B-1细胞不同，脾细胞的DNA结合STAT 3、CREB和PU.1的核表达、IL-10的转录激活以及对PMA的应答不能进入细胞周期。脾B-1细胞（B-1 S）似乎构成了一个独特的B-1细胞群体，虽然与腹膜B-1细胞（B-1 P）共享某些表型特征，但在转录因子和基因表达以及细胞周期进展的信号传导方面与它们不同。(C)2001年，爱思唯尔科学。
B-1 cells constitute a distinct B cell subset with characteristic phenotypic and functional features. B-1 cells are highly represented among peritoneal lymphocytes; substantial numbers of B-1 cells are also located within splenic tissue. Here a number of differences in transcription factor and gene expression were identified that separate peritoneal B-1 and splenic B-2 cells, and then splenic B-1 cells obtained from immunoglobulin transgenic mice were tested for these parameters. Splenic B-1 cells resembled splenic B-2 cells rather than peritoneal B-1 cells in terms of nuclear expression of DNA-binding STAT3, CREB, and PU.1, with respect to transcriptional activation of IL-10, and in the failure to enter cell cycle in response to PMA. Splenic B-1 cells (B-1S) appear to constitute a unique population of B-1 cells, which, while sharing with peritoneal B-1 cells (B-1P) certain phenotypic features, differ from them in transcription factor and gene expression and in signaling for cell cycle progression. (C) 2001 Elsevier Science.