Metal Mesh Device Sensor Immobilized with a Trimethoxysilane-Containing Glycopolymer for Label-Free Detection of Proteins and Bacteria
Metal Mesh Device Sensor Immobilized with a Trimethoxysilane-Containing Glycopolymer for Label-Free Detection of Proteins and Bacteria
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DOI：
10.1021/am503003v
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发表时间：
2014-08-13
影响因子：
9.5
通讯作者：
Miura, Yoshiko
中科院分区：
文献类型：
--
作者：
Seto, Hirokazu;Kamba, Seiji;Miura, Yoshiko
用于检测蛋白质和细菌的生物传感器已经使用二元共聚物固定的金属网装置开发。使用硅烷偶联反应将含三甲氧基硅烷的含糖共聚物固定到金属网装置上。在固定化糖基共聚物后，保持了原始金属网装置的表面形状和透射率特性。甘露糖结合蛋白（伴刀豆球蛋白A）可以检测到浓度范围为10（-9）至10（-6）mol L-1使用glycolmonopolymer固定的金属网装置传感器，而另一种蛋白质（牛血清白蛋白）没有检测到。对吸附的伴刀豆球蛋白A的检测限为1 ng mm（-2）。该聚合物固定的金属网传感器还可以检测细菌以及蛋白质。利用该传感器对甘露糖结合型大肠杆菌进行了特异性检测。因此，该二元共聚物固定的金属网装置可以用作无标记的生物传感器，对蛋白质和细菌显示出高水平的选择性和灵敏度。
Biosensors for the detection of proteins and bacteria have been developed using glycopolymer-immobilized metal mesh devices. The trimethoxysilane-containing glycopolymer was immobilized onto a metal mesh device using the silane coupling reaction. The surface shape and transmittance properties of the original metal mesh device were maintained following the immobilization of the glycopolymer. The mannose-binding protein (concanavalin A) could be detected at concentrations in the range of 10(-9) to 10(-6) mol L-1 using the glycopolymer-immobilized metal mesh device sensor, whereas another protein (bovine serum albumin) was not detected. A detection limit of 1 ng mm(-2) was achieved for the amount of adsorbed concanavalin A. The glycopolymer-immobilized metal mesh device sensor could also detect bacteria as well as protein. The mannose-binding strain of Escherichia coli was specifically detected by the glycopolymer-immobilized metal mesh device sensor. The glycopolymer-immobilized metal mesh device could therefore be used as a label-free biosensor showing high levels of selectivity and sensitivity toward proteins and bacteria.