Fluorescence lifetime imaging in turbid media
Fluorescence lifetime imaging in turbid media
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DOI：
10.1364/ol.21.000158
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发表时间：
1996-01-15
期刊：
影响因子：
3.6
通讯作者：
Yodh, AG
中科院分区：
文献类型：
--
作者：
OLeary, MA;Boas, DA;Yodh, AG
荧光团的寿命通常在不同的环境中变化，使该分子成为组织氧合、pH和葡萄糖的敏感指示剂。然而，当荧光团嵌入在光学厚的、高散射的介质（如人体组织）中时，寿命测量是复杂的。我们制定的逆问题荧光寿命断层扫描使用扩散光子密度波，我们证明了该技术通过推导空间图像的异质荧光团分布和寿命，在异质混浊介质中使用模拟测量。(C)1996年美国光学学会
The lifetime of a fluorophore generally varies in different environments, making the molecule a sensitive indicator of tissue oxygenation, pH, and glucose. However, Lifetime measurements are complicated when the fluorophore is embedded in an optically thick, highly scattering medium such as human tissue. We formulate the inverse problem for fluorescence lifetime tomography using diffuse photon density waves, and we demonstrate the technique by deriving spatial images of heterogeneous fluorophore distribution and Lifetime, using simulated measurements in heterogeneous turbid media. (C) 1996 Optical Society of America