The distribution of topoisomerase II on mammalian chromosomes
The distribution of topoisomerase II on mammalian chromosomes
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拓扑异构酶II在哺乳动物染色体上的分布
DOI：
10.1007/bf02254938
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发表时间：
2005
影响因子：
2.6
通讯作者：
A. T. Sumner
中科院分区：
文献类型：
--
作者：
A. T. Sumner
用免疫荧光技术研究了哺乳动物染色体细胞离心法制备的拓扑异构酶II(Topo II)的分布。Topo II的免疫标记受到固定剂的选择、可及性障碍和酶的不稳定性的影响。仍然嵌入在细胞质中的染色体仍然没有标记，而相比之下，Topo II很容易从没有细胞质的染色体中的一些位置丢失。Topo II的最终分布包括沿每个染色单体中心的一条线，对应于染色体核心或支架，以及染色体上其他地方在有丝分裂过程中变化的Topo II数量。在整个前期染色体中都可以看到Topo II的强烈反应，这与该阶段染色体臂的凝聚和/或分离的作用一致。在中期，Topo II被限制在着丝粒区域，这是染色体在这个阶段仍然需要分离的唯一部分，而在后期，在发生分离后，Topo II的着丝粒浓度丢失。因此，Topo II在哺乳动物染色体中的分布和数量与该酶在染色体凝聚和分离中的已知功能完全一致。
The distribution of topoisomerase II (Topo II) has been studied using immunofluorescence on cytocentrifuged preparations of mammalian chromosomes. Immunolabelling of Topo II is affected by choice of fixative, by barriers to accessibility and by the lability of the enzyme. Chromosomes still embedded in cytoplasm remain unlabelled, while in contrast Topo II can easily be lost from some sites in chromosomes free of cytoplasm. The definitive distribution of Topo II consists of a line along the centre of each chromatid, corresponding to the chromosome core or scaffold, and quantities of Topo II elsewhere in the chromosomes which vary during the course of mitosis. A strong reaction for Topo II can be seen throughout prophase chromosomes, consistent with a role in condensation and/or segregation of the chromosome arms at this stage. At metaphase, Topo II is restricted to the centromeric regions, the only parts of the chromosomes that still have to be separated at this stage, while in anaphase, after segregation has occurred, this centromeric concentration of Topo II is lost. The distribution and quantity of Topo II in mammalian chromosomes is thus wholly consistent with the known functions of this enzyme in chromosome condensation and segregation.
影响因子：
56.9
作者：
TEWEY, KM;ROWE, TC;LIU, LF
通讯作者：
LIU, LF
DOI：
10.1073/pnas.81.9.2616
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DINARDO, S;VOELKEL, K;STERNGLANZ, R
通讯作者：
STERNGLANZ, R