Scale-Up of a Recombinant Pig Liver Esterase-Catalyzed Desymmetrization of Dimethyl Cyclohex-4-ene-cis-1,2-dicarboxylate
Scale-Up of a Recombinant Pig Liver Esterase-Catalyzed Desymmetrization of Dimethyl Cyclohex-4-ene-cis-1,2-dicarboxylate
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重组猪肝酯酶催化环己-4-烯-顺-1,2-二甲酸二甲酯去对称化的放大
DOI：
10.1021/op500129e
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发表时间：
2014
影响因子：
3.4
通讯作者：
U. Kragl
中科院分区：
文献类型：
--
作者：
P. Süss;Sabine Illner;J. Langermann;Sonja Borchert;U. Bornscheuer;Rainer Wardenga;U. Kragl
A recombinant isoenzyme of pig liver esterase was used for the highly enantioselective desymmetrization of dimethyl cyclohex-4-ene-cis-1,2-dicarboxylate. The selected recombinant esterase showed a significant advantage in enantioselectivity over the commonly used esterase from the mammalian source. The process was scaled up to yield 265 g of product with a simplified pH control, and the target molecule was obtained with an enantiopurity of >99.5% ee.
