An unbiased analysis of V(H)-D-J(H) sequences from B-1a, B-1b, and conventional B cells.
An unbiased analysis of V(H)-D-J(H) sequences from B-1a, B-1b, and conventional B cells.
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DOI：
10.4049/jimmunol.158.3.1175
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发表时间：
1997-02
影响因子：
4.4
通讯作者：
Aaron B. Kantor;C. Merrill;L. Herzenberg;J. Hillson
中科院分区：
文献类型：
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作者：
Aaron B. Kantor;C. Merrill;L. Herzenberg;J. Hillson
先前的研究得出结论，B-1a （CD5+ B）细胞的库受到高度限制。这里的研究使用FACS分选和单细胞PCR方法对来自腹腔的B-1a、B-1b和传统B细胞的IgH谱进行了无偏表示，结果表明B-1a细胞谱比以前认为的更加多样化。此外，成年B-1a细胞的非编码核苷酸(N)插入明显少于常规B细胞。然而，B-1a细胞并不是由这些区域的缺失来定义的，因为这些插入存在于三分之二的B-1a细胞转录本中。所有三种B细胞群都使用广泛的V(H)、D和J(H)元素，并且在互补决定区3 （CDR3）中显示出相当大的多样性。然而，这三种B细胞群的功能库也存在特征差异，这表明不同的选择和/或发育力量决定了每种功能库的形成。
Previous studies conclude that the repertoire of B-1a (CD5+ B) cells is highly restricted. Studies here, which use FACS sorting and single-cell PCR methodology to develop an unbiased representation of the IgH repertoires of B-1a, B-1b, and conventional B cells from the peritoneal cavity, demonstrate that the B-1a cell repertoire is more diverse than previously thought. Furthermore, adult B-1a cells have significantly fewer noncoded nucleotide (N) insertions than conventional B cells. However, B-1a cells are not defined by the absence of these regions, since such insertions are present in two-thirds of B-1a cell transcripts. All three B cell populations use a wide spectrum of V(H), D, and J(H) elements and display considerable diversity in complementarity-determining region 3 (CDR3). However, characteristic differences in the repertoires of all three B cell populations also exist, suggesting different selective and/or developmental forces act to shape each repertoire.