p73 and reelin in Cajal-Retzius cells of the developing human hippocampal formation
p73 and reelin in Cajal-Retzius cells of the developing human hippocampal formation
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DOI：
10.1093/cercor/bhh010
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发表时间：
2004-05-01
期刊：
影响因子：
3.7
通讯作者：
Meyer, G
中科院分区：
文献类型：
--
作者：
Abraham, H;Pérez-García, CG;Meyer, G
在胚胎人的海马区，Cajal-Retzius(CR)细胞共表达P73和糖蛋白reelin，P73是参与细胞存活和凋亡的家族成员，而糖蛋白reelin对径向迁移至关重要。我们区分了两类可能的CR细胞。(1)p73/reelin表达细胞在10周左右出现在侧脑室颞角皮质脉络膜交界处(腹侧皮质下缘)，占据位于氨质原基和齿状原基之上的边缘带(MZ)。(2)从14GW开始，在齿状-海马伞边界附近的神经上皮细胞中出现额外的p73阳性细胞，并向硬膜表面扩散，位于迁移的次生齿状基质的两侧。13~17GW时，齿状回的大部分几乎没有CR细胞。16GW时，p73/Reelin阳性的CR细胞出现在锥体上叶的MZ，21GW左右的锥体下叶出现。齿状-海马伞交界处的p73阳性细胞在靠近硬膜表面时表达卷曲，提示它们分化为锥体下叶的CR细胞。早在14GW时，Reelin阳性、p73阴性的中间神经元就出现在预期的羊角陷窝分子层和放射层；在齿状分子层和门部，它们出现在中期左右。我们认为人海马结构中的CR细胞属于两个不同的细胞群：早期的CR细胞来自腹侧皮质，主要与氨板和齿状板的迁移有关，而较晚出现的CR细胞来自齿状海马伞神经上皮，这可能与齿状颗粒细胞，特别是锥体下叶的长时间神经发生和迁移有关。
In the fetal human hippocampus, Cajal-Retzius (CR) cells coexpress p73, a p53-family member involved in cell survival and apoptosis, and the glycoprotein reelin, crucial for radial migration. We distinguish two populations of putative CR cells. (1) p73/reelin expressing cells appear around 10 gestational weeks (GW) at the cortico-choroid border in the temporal horn of the lateral ventricle (the ventral cortical hem) and occupy the marginal zone (MZ) overlying the ammonic and dentate primordia. (2) Additional p73-positive cells appear from 14 GW onward in the neuroepithelium near the dentate-fimbrial boundary and spread toward the pial surface, flanking the migrating secondary dentate matrix. From 13 to 17 GW, large parts of the dentate gyrus are almost devoid of CR cells. p73/Reelin-positive CR cells appear in the MZ of the suprapyramidal blade at 16 GW and around 21 GW in the infrapyramidal blade. The p73-positive cells of the dentate-fimbrial boundary express reelin when they are close to the pial surface, suggesting that they differentiate into CR cells of the infrapyramidal blade. Reelin-positive, p73-negative interneurons are prominent in the prospective strata lacunosum-moleculare and radiatum of cornu ammonis as early as 14 GW; in the dentate molecular layer and hilus they appear around midgestation. We propose that CR cells of the human hippocampal formation belong to two distinct cell populations: an early one derived from the ventral cortical hem and mainly related to migration of the ammonic and dentate plates and a later appearing one derived from the dentate-fimbrial neuroepithelium, which may be related to the protracted neurogenesis and migration of dentate granule cells, particularly of the infrapyramidal blade.