A unique cell-surface protein phenotype distinguishes human small-cell from non-small-cell lung cancer.
A unique cell-surface protein phenotype distinguishes human small-cell from non-small-cell lung cancer.
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独特的细胞表面蛋白表型将人类小细胞肺癌与非小细胞肺癌区分开来。
DOI：
10.1073/pnas.79.15.4650
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发表时间：
1982
影响因子：
11.1
通讯作者：
Shaper,JH
中科院分区：
文献类型：
--
作者：
Baylin,SB;Gazdar,AF;Minna,JD;Bernal,SD;Shaper,JH
我们已经使用放射性碘标记（125 I）和二维聚丙烯酰胺凝胶电泳，以确定小（燕麦）细胞肺癌（SCC）-一种具有神经内分泌功能的肿瘤-具有不同于其他类型的肺癌细胞（鳞状，腺，和大细胞未分化癌）的表面蛋白模式。12个不同的蛋白质，40至70千道尔顿（kDal），特点是四个独立的线SCC;其中三个，指定E（60 kDal; pI = 7.3），S（30 kDal; pI = 6.0），和U（57 kDal; pI = 5.6），可能是独特的SCC基因产物，并确定只有在[35 S]蛋氨酸标记的SCC，而不是在非SCC或人成纤维细胞。两个线的腺，一个鳞状细胞癌，和未分化的大细胞肺癌表现出相似的表面蛋白模式彼此。9个区别蛋白（40至100 kDal）和至少5个大蛋白（大于100 kDal）是这些系所特有的。SCC和非SCC的表面蛋白表型与人类淋巴母细胞和成纤维细胞的表面蛋白表型不同。然而，SCC的神经内分泌特征得到了进一步证实，因为12种区分SCC表面蛋白中的6种，包括E和U，在人神经母细胞瘤细胞上被鉴定。所鉴定的蛋白质应（i）有助于定义正常和肿瘤性支气管上皮细胞的分化步骤，（ii）证明可用于更好地分类肺癌，以及（iii）有助于追踪神经内分泌细胞的形成。
We have used radioiodination (125I) and two-dimensional polyacrylamide gel electrophoresis to determine that small- (oat) cell lung carcinoma (SCC)--a tumor with neuroendocrine features--possesses a surface protein pattern distinct from the other types of lung cancer cells (squamous, adeno-, and large-cell undifferentiated carcinoma). Twelve distinguishing proteins, 40 to 70 kilodaltons (kDal), characterized four separate lines of SCC; three of these, designated E (60 kDal; pI = 7.3), S (30 kDal; pI = 6.0), and U (57 kDal; pI = 5.6), may be unique SCC gene products and were identified only in [35S]methionine labeling of SCC and not in non-SCC or human fibroblasts. Two lines of adeno-, one of squamous, and one of undifferentiated large-cell lung carcinoma exhibited similar surface protein patterns to one another. Nine distinguishing proteins (40 to 100 kDal) and at least five large proteins (greater than 100 kDal) were unique to these lines. The surface protein phenotypes for SCC and non-SCC were distinct from those for human lymphoblastoid cells and fibroblasts. However, the neuroendocrine features of SCC were further substantiated because 6 of the 12 distinguishing SCC surface proteins, including E and U, were identified on human neuroblastoma cells. The proteins identified should (i) help define differentiation steps for normal and neoplastic bronchial epithelial cells, (ii) prove useful in better classifying lung cancers, and (iii) be instrumental in tracing formation of neuroendocrine cells.