Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor
Involvement of pituitary gonadotropins, gonadal steroids and breeding season in sex change of protogynous dusky grouper, Epinephelus marginatus (Teleostei: Serranidae), induced by a non-steroidal aromatase inhibitor
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DOI：
10.1016/j.ygcen.2013.06.012
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发表时间：
2013-10-01
影响因子：
2.7
通讯作者：
Moreira, Renata Guimaraes
中科院分区：
文献类型：
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作者：
Garcia, Carlos Eduardo de O.;Araujo, Bruno C.;Moreira, Renata Guimaraes
用芳香酶抑制剂(AI)来曲唑(100 mg/kg)促进雌性暗斑石斑鱼由雌变雄进行了两次实验。一个实验是在繁殖季节(春季)进行的，另一个实验是在繁殖季节结束时(夏季)进行的。在春季，人工智能促进了9周后的性别变化，产生的精子能够使石斑鱼卵母细胞受精。在夏天，变性是不完整的；出现了双性个体，任何动物都没有释放精子。变性的性腺具有板层结构，生殖室的管腔内有精母细胞和精子的囊。春季，4周后，AI组11-酮-睾酮(11kT)水平高于对照鱼，9周后，随着精液释放，AI组睾酮水平升高，而11-KT水平恢复到初始水平。雌二醇(E-2)水平在实验期间保持不变。而不是像对照组那样在整个过程中下降，人工智能处理的鱼的17α-羟孕酮水平没有变化，导致9周后与对照鱼相比值更高。9周后AI组FSHβ和LHβ基因表达水平均低于对照鱼。人工智能的使用是在繁殖期获得功能性雄性的有效方法。在促性腺激素的调节下，雄激素的增加触发了性别变化，使雄性生殖细胞得以发育，而暗色石斑鱼并不需要E-2水平的降低来改变性别。(C)2013 Elsevier Inc.保留所有权利。
Two experiments were performed using the aromatase inhibitor (AI) letrozole (100 mg/kg) to promote sex change, from female-to-male, in protogynous dusky grouper. One experiment was performed during the breeding season (spring) and the other at the end of the breeding season (summer). During the spring, AI promoted sex change after 9 weeks and the sperm produced was able to fertilize grouper oocytes. During the summer, the sex change was incomplete; intersex individuals were present and sperm was not released by any of the animals. Sex changed gonads had a lamellar architecture; cysts of spermatocytes and spermatozoa in the lumen of the germinal compartment. In the spring, after 4 weeks, 11ketotestosterone (11KT) levels were higher in the AI than in control fish, and after 9 weeks, coincident with semen release, testosterone levels increased in the AI group, while 11 KT returned to the initial levels. Estradiol (E-2) levels remained unchanged during the experimental period. Instead of decreasing throughout the period, as in control group, 17 alpha-OH progesterone levels did not change in the AI-treated fish, resulting in higher values after 9 weeks when compared with control fish. fsh beta and lh beta gene expression in the AI animals were lower compared with control fish after 9 weeks. The use of AI was effective to obtain functional males during the breeding season. The increase in androgens, modulated by gonadotropins, triggered the sex change, enabling the development of male germ cells, whereas a decrease in E-2 levels was not required to change sex in dusky grouper. (C) 2013 Elsevier Inc. All rights reserved.