Bioguided Purification of Active Compounds from Leaves of Anadenanthera colubrina var. cebil (Griseb.) Altschul
Bioguided Purification of Active Compounds from Leaves of Anadenanthera colubrina var. cebil (Griseb.) Altschul
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DOI：
10.3390/biom9100590
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发表时间：
2019-10-01
期刊：
影响因子：
5.5
通讯作者：
dos Santos Correia, Maria Tereza
中科院分区：
文献类型：
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作者：
Cavalcante de Araujo, Daniel Rodrigo;da Silva, Tulio Diego;dos Santos Correia, Maria Tereza
天南星Andenanthera colubrina var cebil(Griseb.)Altschul是一种药用植物，遍布巴西半干旱地区。本工作在生物引导下对白蜡梅叶乙酸乙酯提取物中存在的活性物质进行了纯化。抗金黄色葡萄球菌和抗氧化作用作为生物活性的标志。对提取物进行闪层析，得到五个组分(F1、F2、F3、F4和F5)。组分F2和F4的抑菌活性最强，对金黄色葡萄球菌UFPEDA 02的半数抑菌浓度(IN50)为19.53mU g/mL，而F4对DPPH自由基(2，2-二苯基-1-苦基肼)的抑制作用较强[抑制50%自由基的剂量(IC50)=133+/-9mU/mL]。对F2和F4进行制备性高效液相色谱和核磁共振分析，确定F2和F4中的主要成分为对羟基苯甲酸和金丝桃苷。金丝桃苷和对羟基苯甲酸对金黄色葡萄球菌UFPEDA 02的半数抑菌浓度分别为250微克/毫升和500微克/毫升。金丝桃苷对临床分离的多药耐药金黄色葡萄球菌UFPEDA 705的IN50为62.5微克/毫升。在纯化的化合物中，F2得到的原花青素具有较高的抗氧化能力。综上所述，这些结果突出了白头翁树叶作为制药、食品和化妆品行业感兴趣的生物分子的替代来源的潜力。
Anadenanthera colubrina var cebil (Griseb.) Altschul is a medicinal plant found throughout the Brazilian semi-arid area. This work performed a bioguided purification of active substances present in ethyl acetate extract from A. colubrina leaves. The anti-Staphylococcus aureus and antioxidant actions were used as markers of bioactivity. The extract was subjected to flash chromatography resulting in five fractions (F1, F2, F3, F4, and F5). The fractions F2 and F4 presented the highest antimicrobial action, with a dose able to inhibit 50% of bacteria growth (IN50) of 19.53 mu g/mL for S. aureus UFPEDA 02; whereas F4 showed higher inhibitory action towards DPPH radical (2,2-diphenyl-1-picryl-hydrazyl-hydrate) [dose able to inhibit 50% of the radical (IC50) = 133 +/- 9 mu g/mL]. F2 and F4 were then subjected to preparative high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR), resulting in the identification of p-hydroxybenzoic acid and hyperoside as the major compounds in F2 and F4, respectively. Hyperoside and p-hydroxybenzoic acid presented IN50 values of 250 mu g/mL and 500 mu g/mL against S. aureus UFPEDA 02, respectively. However, the hyperoside had an IN50 of 62.5 mu g/mL against S. aureus UFPEDA 705, a clinical isolate with multidrug resistant phenotype. Among the purified compounds, the proanthocyanidins obtained from F2 exhibited the higher antioxidant potentials. Taken together, these results highlight the potential of A. colubrina leaves as an alternative source of biomolecules of interest for the pharmaceutical, food, and cosmetic industries.