Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart
Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart
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离体晶体灌注大鼠心脏中的钠 TQF NMR 和细胞内钠
DOI：
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发表时间：
1998
影响因子：
3.3
通讯作者：
J. N. Young
中科院分区：
文献类型：
--
作者：
V. Schepkin;I. O. Choy;T. Budinger;D. Y. Obayashi;Scott E. Taylor;W. DeCampli;S. Amartur;J. N. Young
The feasibility of monitoring intracellular sodium changes using Na triple quantum filtered NMR without a chemical shift reagent (SR) was investigated in an isolated rat heart during a variety of interventions for Ma, loading. Perfusion with 1 mM ouabain or without K+ present in the perfusate for 30 min produced a rise of the Na TQF signal with a plateau of ‐190% and ˜228% relative to the preintervention level, respectively. Stop‐flow ischemia for 30 min resulted in a TQF signal growth of ˜147%. The maximal Na TQF signal increase of 460% was achieved by perfusion without K+/Ca2+, corresponding to an elimination of the Na transmembrane gradient. The observed values of Na NMR TQF growth in the physiological and pathological ranges are in agreement with reported data by other methods and have a linear correlation with intracellular sodium content as determined in this study by Co‐EDTA method and by sucrose‐histidine washout of the extracellular space. Our data indicate that the increase in Na TQF NMR signal is determined by the growth of Nai, and the extracellular Na contribution to the total TQF signal is unchanged at ˜64%. In conclusion, Na TQF NMR without using SR offers a unique and noninvasive opportunity to monitor alterations of intracellular sodium. It may provide valuable insights for developing car‐dioprotective strategies and for observing the effects of pharmaceutical treatments on sodium homeostasis.
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Langendorff 灌注大鼠心脏的多核核磁共振研究。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jelicks,LA;Gupta,RK
通讯作者：
Gupta,RK
核磁共振测量正常血压和自发性高血压大鼠心脏细胞内钠。
影响因子：
3.2
作者：
Jelicks,LA;Gupta,RK
通讯作者：
Gupta,RK
心脏停跳期间离体大鼠心脏中钠的变化。
DOI：
10.1152/jappl.1996.81.6.2696
发表时间：
1996
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Schepkin,VD;Choy,IO;Budinger,TF
通讯作者：
Budinger,TF
灌注大鼠心脏的 23Na 和 39K 核磁共振研究。
影响因子：
3.4
作者：
Pike,MM;Frazer,JC;Dedrick,DF;Ingwall,JS;Allen,PD;SpringerJr,CS;Smith,TW
通讯作者：
Smith,TW