Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents
Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents
复制标题
DOI：
10.1364/boe.9.002229
复制
发表时间：
2018-05-01
影响因子：
3.4
通讯作者：
Razansky, Daniel
中科院分区：
文献类型：
--
作者：
Chen, Zhenyue;Dean-Ben, Xose Luis;Razansky, Daniel
荧光造影剂广泛用于生物医学研究。虽然许多研究已经报道了使用基于荧光或基于吸收（光声）成像系统的荧光部分的深部组织成像，但是没有关于这些成像模式在检测深层荧光剂中的实际性能进行系统比较。在本文中，已经采用了结合落射荧光和体积光声成像能力的集成成像器，以便在两种模式下评估几种常用的近红外染料的图像随深度的退化。我们在含有填充有不同浓度的Alexa Fluor 700、Alexa Fluor 750、吲哚菁绿色（ICG）和IRDye 800 CW的深包埋靶的仿组织体模中进行对照实验。ICG通过小鼠体内组织的多模态成像进一步证实了这一结果。结果表明，光声始终提供更好的灵敏度，在区分荧光目标位于超过2毫米的深度混浊组织，量化评估图像对比度，信噪比和空间分辨率性能。(c)根据OSA开放获取出版协议的条款，2018年美国光学学会。
Fluorescent contrast agents are widely employed in biomedical research. While many studies have reported deep tissue imaging of fluorescent moieties using either fluorescence-based or absorption-based (optoacoustic) imaging systems, no systematic comparison has been performed regarding the actual performance of these imaging modalities in detecting deep-seated fluorescent agents. Herein, an integrated imager combining epi-fluorescence and volumetric optoacoustic imaging capabilities has been employed in order to evaluate image degradation with depth for several commonly-used near-infrared dyes in both modes. We performed controlled experiments in tissue-mimicking phantoms containing deeply embedded targets filled with different concentrations of Alexa Fluor 700, Alexa Fluor 750, indocyanine green (ICG) and IRDye 800CW. The results are further corroborated by multi- modal imaging of ICG through mouse tissues in vivo. It is shown that optoacoustics consistently provides better sensitivity in differentiating fluorescent targets located at depths beyond 2 mm in turbid tissues, as quantified by evaluating image contrast, signal to noise ratio and spatial resolution performance. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.