Comparative studies of muscarinic and dopamine receptors in three strains of rat.
Comparative studies of muscarinic and dopamine receptors in three strains of rat.
复制标题
三种大鼠品系毒蕈碱和多巴胺受体的比较研究。
DOI：
10.1016/0014-2999(89)90722-x
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发表时间：
1989
影响因子：
5
通讯作者：
Ho,IK
中科院分区：
文献类型：
--
作者：
Lim,DK;Ito,Y;Hoskins,B;Rockhold,RW;Ho,IK
本文研究了9周龄雄性SD、WKY和自发性高血压大鼠（SHR）胆碱酯酶（ChE）活性及胆碱能和多巴胺的特性。血浆胆碱酯酶活性在WKY显着低于（50%）比其他strians的活动。在毒蕈碱受体的研究中，SD大鼠纹状体[~ 3 H]QNB结合位点的数目（18%）低于WKY和SHR。然而，毒蕈碱受体的特性（Kd和Bmax）在下丘脑是相同的。多巴胺受体的研究表明，在纹状体和下丘脑的D-1和D-2受体的密度显着高于SHR比其他品系。然而，在亲和力常数（Kd）上没有差异。自发性高血压大鼠下丘脑的高密度主要是由于下丘脑后部D-α和D-2受体的高密度。在下丘脑前部，D-2受体的数量没有差异。这些结果提供了一个实质性的基础，即药物代谢酶和受体群体的改变，在此基础上建立对外源性物质作用的遗传易感性的理解。
Cholinesterase activities and characteristics of muscarinic and dopamine form 9 week old male Sprague-Dawley (SD), Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR) were studied. Plasma cholinesterase activity in WKY was significantly lower (50%) than activity in the other strians. In studies of muscarinic receptors, the number of [3H]QNB binding sites in striata from SD rats was lower (18%) than those from WKY and SHR. However, muscarinic receptor properties (Kdand Bmax) were the same in hypothalami. Studies of dopamine receptors revealed that the densities of both D-1 and D-2 receptors in both striata and hypothalami were significantly higher in SHR than in other strains. However, there were no differences in the affinity constant (Kd). The higher densities in hypothalami from SHR were mainly due to the high populaion of D-a and D-2 receptors in the posterior hypothalamus. In the anterior hypothalamus, there was no difference in the population of D-2 receptors. These results provide a substantive basis, i.e. demonstration of alterations in drug metabolizing enzymes and receptor populations, on which to build an understanding of the genetic predisposition to the actions of xenobiotic agents.