Repair of Large Osteochondral Defects in Rabbits Using Porous Hydroxyapatite/Collagen (HAp/Col) and Fibroblast Growth Factor-2 (FGF-2)
Repair of Large Osteochondral Defects in Rabbits Using Porous Hydroxyapatite/Collagen (HAp/Col) and Fibroblast Growth Factor-2 (FGF-2)
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DOI：
10.1002/jor.21032
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发表时间：
2010-05-01
影响因子：
2.8
通讯作者：
Okawa, Atsushi
中科院分区：
文献类型：
--
作者：
Maehara, Hidetsugu;Sotome, Shinichi;Okawa, Atsushi
关节软骨的自我更新能力有限。本文报道了多孔羟基磷灰石/胶原 (HAp/Col) 支架作为骨空隙填充剂和药物给药载体的开发。该支架由 HAp 纳米晶体和 I 型去端胶原组成。本研究的目的是研究浸渍 FGF-2 的多孔 HAp/Col 修复兔模型中大骨软骨缺损的功效。在右膝股骨滑车沟内制作96个直径5毫米、深度5毫米的圆柱形骨软骨缺损。将动物分配到四个处理组之一：用50μl浓度为10或100μg/ml的FGF-2浸渍的多孔HAp/Col(FGF10或FGF100组)；多孔HAp/Col，含50μl PBS（HAp/Col组）；并且没有植入（缺陷组）。对缺损区域进行肉眼和组织学检查。术后 3、6、12 和 24 周对软骨下骨再生进行量化。与缺陷组相比，HAp/Col 植入组观察到丰富的骨形成。 FGF10组不仅表现出最丰富的骨再生，而且软骨再生也最令人满意，软骨呈现透明样外观。这些发现表明，多孔 HAp/Col 与 FGF-2 增强了软骨修复过程。 (C) 2009 年骨科研究学会。出版者：Wiley periodicals, Inc. J Orthop Res 28:677-686, 2010
Articular cartilage has a limited capacity for self-renewal. This article reports the development of a porous hydroxyapatite/collagen (HAp/Col) scaffold as a bone void filler and a vehicle for drug administration. The scaffold consists of HAp nanocrystals and type I atelocollagen. The purpose of this study was to investigate the efficacy of porous HAp/Col impregnated with FGF-2 to repair large osteochondral defects in a rabbit model. Ninety-six cylindrical osteochondral defects 5 mm in diameter and 5 mm in depth were created in the femoral trochlear groove of the right knee. Animals were assigned to one of four treatment groups: porous HAp/Col impregnated with 50 mu l of FGF-2 at a concentration of 10 or 100 mu g/ml (FGF10 or FGF100 group); porous HAp/Col with 50 mu l of PBS (HAp/Col group); and no implantation (defect group). The defect areas were examined grossly and histologically. Subchondral bone regeneration was quantified 3, 6, 12, and 24 weeks after surgery. Abundant bone formation was observed in the HAp/Col implanted groups as compared to the defect group. The FGF10 group displayed not only the most abundant bone regeneration but also the most satisfactory cartilage regeneration, with cartilage presenting a hyaline-like appearance. These findings suggest that porous HAp/Col with FGF-2 augments the cartilage repair process. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:677-686, 2010