Fumigant activity of volatile compounds of Streptomyces philanthi RM-1-138 and pure chemicals (acetophenone and phenylethyl alcohol) against anthracnose pathogen in postharvest chili fruit
Fumigant activity of volatile compounds of Streptomyces philanthi RM-1-138 and pure chemicals (acetophenone and phenylethyl alcohol) against anthracnose pathogen in postharvest chili fruit
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DOI：
10.1016/j.cropro.2017.09.002
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发表时间：
2018-01-01
期刊：
影响因子：
2.8
通讯作者：
Prasertsan, Poonsuk
中科院分区：
文献类型：
--
作者：
Boukaew, Sawai;Petlamul, Wanida;Prasertsan, Poonsuk
以无菌小麦种子为材料，研究了甜链霉菌Rm-1-138的挥发物(Rm-1-138)和商品纯挥发物(苯乙酮和苯乙醇)对辣椒炭疽病的抑制作用。从42个炭疽菌菌株中筛选出对挥发物RM-1-138最具侵袭力的炭疽菌菌株PSU-NY8。甜链霉菌Rm-1-138种子培养的最适接种量(15g L-1)和最适孢子浓度(107个孢子mL-1)对球孢霉PsU-NY8有完全抑制作用(100%抑制)。此外，体内试验表明，挥发物Rm-1-138(15g L-1)的最佳熏蒸时间为6h，苯乙醇在所测试的所有浓度(10-10001.1 L-1)下均无抑菌活性，而苯乙酮在100~1.11 L-1的空气中熏蒸12h后在体内外均表现出抗真菌活性。扫描电子显微镜下可见球孢子菌PSU-NY8的细胞壁被破坏，证明了其熏蒸活性。因此，甜椒菌株Rm-1-138的挥发物在辣椒炭疽病的采后生物防治中具有较高的潜力。(C)2017爱思唯尔有限公司。保留所有权利。
Application of volatiles from Streptomyces philanthi RM-1-138 grown on sterile wheat seeds (volatiles RM-1-138) and pure commercial volatile compounds (acetophenone and phenylethyl alcohol) against chili anthracnose pathogen were investigated in vitro and in vivo. Among 42 isolates of Colletotrichum species tested, C gloeosporioides PSU-NY8 was selected as the most aggressive anthracnose pathogenic strain on chili fruit against the volatiles RM-1-138. The optimum inoculum size (15 g L-1) and spore concentration (107 spore mL(-1)) of the wheat seed culture of S. philanthi RM-1-138 exhibited the complete suppression (100% inhibition) on C gloeosporioides PSU-NY8. In addition, the in vivo result indicated the optimum fumigation period of the volatiles RM-1-138 (15 g L-1) was at 6 h. Phenylethyl alcohol showed no antifungal activity at all concentrations tested (10-1000 1.1 L-1) while acetophenone at 100 1.11 L-1 of airspace exhibited antifungal activity both in vitro and in vivo after 12 h fumigation. The fumigant activity was evidenced by the damage of cell wall of C gloeosporioides PSU-NY8 illustrated by SEM. Therefore, the volatiles from S. philanthi RM-1-138 have a high potential for biocontrol of chili anthracnose disease in postharvest system. (C) 2017 Elsevier Ltd. All rights reserved.