Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID.
Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID.
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Sp1 按照 TATA 或启动子的指导进行转录激活：对哺乳动物转录因子 IID 的特定要求。
DOI：
10.1073/pnas.87.12.4509
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发表时间：
1990
影响因子：
11.1
通讯作者：
Baltimore,D
中科院分区：
文献类型：
--
作者：
Smale,ST;Schmidt,MC;Berk,AJ;Baltimore,D
RNA聚合酶II对哺乳动物基因的转录通常始于特定的核苷酸，其位置由称为TATA盒的上游DNA元件或位于转录起始位点的称为起始子（Inr）的元件决定。通过合成启动子的体外分析，我们在这里证明TATA和Inr元件在功能上是相似的，并且Inr包含在相对于起始位点的核苷酸-3和+5之间。此外，我们发现，哺乳动物转录因子IID（TFIID）的蛋白组分是转录刺激所需的Sp1依赖性激活元件放置在上游的TATA或Inr元件。然而，在这些试验中，酵母TATA结合蛋白，这是以前被证明功能类似于哺乳动物TFIID，不能有效地替代哺乳动物TFIID部分。这些结果表明，哺乳动物TFIID是从酵母TATA结合蛋白的功能不同，并可能含有额外的亚基或结构域，从一些启动子的转录激活是重要的。
Transcription of mammalian genes by RNA polymerase II often begins at a specific nucleotide, whose location is determined either by an upstream DNA element known as a TATA box or by an element positioned at the transcription start site called an initiator (Inr). By in vitro analysis of synthetic promoters, we demonstrate here that the TATA and Inr elements are functionally similar and that the Inr is contained between nucleotides -3 and +5 relative to the initiation site. Moreover, we found that a mammalian transcription factor IID (TFIID) protein fraction is required for transcriptional stimulation by an Sp1-dependent activating element placed upstream of either TATA or Inr elements. However, in these assays, the yeast TATA-binding protein, which previously was shown to function similarly to mammalian TFIID, could not efficiently substitute for the mammalian TFIID fraction. These results demonstrate that mammalian TFIID is functionally distinct from the yeast TATA-binding protein and may contain additional subunits or domains that are important for transcriptional activation from some promoters.