TISSUE DISTRIBUTION OF LYMPHOCYTES IN RHEUMATIC HEART-VALVES AS DEFINED BY MONOCLONAL ANTI-T-CELL ANTIBODIES
TISSUE DISTRIBUTION OF LYMPHOCYTES IN RHEUMATIC HEART-VALVES AS DEFINED BY MONOCLONAL ANTI-T-CELL ANTIBODIES
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DOI：
10.1016/0002-9343(83)91124-5
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发表时间：
1983-01-01
影响因子：
5.9
通讯作者：
HUSBY, G
中科院分区：
文献类型：
--
作者：
RAIZADA, V;WILLIAMS, RC;HUSBY, G
采用常规组织学染色和间接免疫荧光技术，对106例印度风湿性心脏病患者在心脏矫正手术时切除的新鲜心脏瓣膜组织和心房附件进行检查，以确定瓣膜间质淋巴细胞浸润的特征。使用抗igg、抗ia和单克隆小鼠杂杂瘤试剂鉴定T细胞（OKT3）和T细胞亚群（OKT4辅助/诱导剂和OKT8抑制/细胞毒性细胞），试图精确鉴定炎症单核细胞的表型特征。在Albuquerque [New Mexico， USA]接受心脏瓣膜切除术的21例患者进行了类似的研究。印度患者提供瓣膜组织的平均年龄为27.7岁；在阿尔伯克基，这个数字是52岁。25%的印度风湿性心脏瓣膜患者表现出明显的间质性淋巴浸润；阿尔伯克基1/3患者的风湿性瓣膜显示类似的单核细胞集合。淋巴浸润以T细胞为主（70-80%），偶有B细胞。大多数T细胞为okt4阳性，只有少量抑制/细胞毒性okt8阳性T细胞。在许多情况下，okt4阳性辅助T细胞收集与成纤维细胞和胶原原纤维紧密并列。显然，慢性风湿性瘢痕形成过程涉及辅助/诱导剂T细胞作为一个辅助因素在变形的心脏瓣膜结构的无痛挛缩和纤维化。使用广泛的异种兔F(ab ")2试剂，对a组链球菌的膜、细胞壁粘肽或a组碳水化合物具有特异性，试图通过间接免疫荧光来证明残留的链球菌抗原，结果为阴性。
Fresh cardiac valvular tissues and atrial appendages removed from 106 Indian patients with rheumatic heart disease at the time of corrective cardiac surgery were examined to determine the characteristics of valvular interstitial lymphocytic infiltrates using conventional histologic staining along with indirect immunofluorescent techniques. Precise identification of the phenotypic profiles of inflammatory mononuclear cells was attempted using anti-IgG, anti-Ia, and monoclonal mouse hybridoma reagents identifying T cells (OKT3) and T cell subsets (OKT4 helper/inducer and OKT8 suppressor/cytotoxic cells). A similar group of 21 patients undergoing cardiac valvular resection in Albuquerque [New Mexico, USA] was studied. The mean age of Indian patients providing valve tissues was 27.7; in those in Albuquerque, it was 52 yr. Twenty-five percent of rheumatic heart valves in Indian patients showed significant interstitial lymphoid infiltrates; 1/3 of the rheumatic valves from patients in Albuquerque showed similar mononuclear cell collections. Lymphoid infiltrates contained a predominance of T cells (70-80%) and only occasional B cells. Most of the T cells were OKT4-positive with only a minor representation of suppressor/cytotoxic OKT8-positive T cells. In many instances, OKT4-positive helper T cell collections were closely juxtaposed to fibroblasts and collagen fibrils. Apparently, the chronic rheumatic scarring process involves helper/inducer T cells as an ancillary factor in the indolent contracture and fibrosis of deformed cardiac valvular structures. Attempts to demonstrate residual streptococcal antigens by indirect immunofluorescence using a wide panel of heterologous rabbit F(ab'')2 reagents with specificity for group A streptococcal membranes, cell wall mucopeptide or group A carbohydrate gave negative results.