Studies on the mechanism of entry of vaccinia virus in animal cells
Studies on the mechanism of entry of vaccinia virus in animal cells
复制标题
痘苗病毒侵入动物细胞机制的研究
作者：
R. Janeczko;J. F. Rodríguez;M. Esteban
摘要为了研究痘苗病毒进入细胞的机制，在用 35 S-甲硫氨酸标记的病毒感染非洲绿猴肾（BSC-40）细胞期间研究了病毒颗粒相关多肽的命运。感染后 3 小时，大约 12-15% 的病毒粒子多肽被降解为酸溶性产物。蛋白水解被甲胺抑制（50%），表明降解位点是溶酶体。甲胺或氯喹均不抑制病毒感染性或脱壳，表明非酸性内吞机制的进入。密度梯度的亚细胞分级分离研究表明，大部分输入的病毒粒子多肽与质膜级分相关。此外，输入的病毒粒子 DNA 部分地从膜部分中分离出来。结果与涉及病毒与质膜融合的进入机制最为一致。
SummaryIn order to study the mechanism of entry of vaccinia virus into cells the fate of virion associated polypeptides was investigated during infection of african green monkey kidney (BSC-40) cells with 35 S-methionine labelled virus. Approximately 12–15 percent of the virion polypeptides were degraded to acid-soluble products by 3 hours post-infection. Proteolysis was inhibited (50 percent) by methylamine, suggesting a lysosomal site of degradation. Neither methylamine or chloroquine inhibited virus infectivity or uncoating indicating a non-acid endocytic mechanism of entry. Subcellular fractionation studies on density gradients indicated that the bulk of the input virion polypeptides were associated with the plasma membrane fraction. In addition, input virion DNA was partially resolved from the membrane fraction. The results are most consistent with a mechanism of entry involving fusion of the virus with the plasma membrane.
将大鼠肝脏的生物活性膜蛋白插入小鼠成纤维细胞的质膜。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Baumann,H;Hou,E;Doyle,D
通讯作者：
Doyle,D