Hepatic arterial perfusion regulates portal venous flow between hepatic sinusoids and intrahepatic shunts in the normal rat liver in vitro
Hepatic arterial perfusion regulates portal venous flow between hepatic sinusoids and intrahepatic shunts in the normal rat liver in vitro
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DOI：
10.1007/s004240100682
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发表时间：
2001-11-01
影响因子：
4.5
通讯作者：
Naftalin, R
中科院分区：
文献类型：
--
作者：
Alexander, B;Cottam, H;Naftalin, R
肝内分流调节门静脉压力在急性门静脉高压症期间，当跨鞘门静脉阻力瞬间增加在正常大鼠肝脏在体内。肝动脉流入也可增加经肝门静脉阻力，激活肝内分流。本研究测定了离体大鼠经肝门静脉阻力和肝内分流的活性，并确定了大鼠肝动脉和门静脉的汇合点。雄性Sprague-Dawley大鼠的肝脏在体外进行单向双灌注。完全停止或分流肝动脉流入门静脉血管系统，同时维持总肝灌注液流量。降低窦内压力，增加经肝门静脉阻力，并以类似于门静脉内注射15 μ m直径微球的方式打开门静脉肝内分流。将微球注射到肝动脉循环中显著增加了肝动脉压，与动脉血管系统的完全闭塞一致。肝内分流位于窦前水平，因为门静脉内注射微球后未检测到肝动脉压升高。与门静脉供血不同，肝动脉血管系统不具有侧支分流循环，并在窦内位置与门静脉合并。
Intrahepatic shunts regulate portal venous pressure during periods of acute portal hypertension when the transhepatic portal resistance is momentarily increased in the normal rat liver in vivo. Hepatic arterial inflow may also increase the transhepatic portal resistance and activate intrahepatic shunts. In the present study, the transhepatic portal resistance and the activity of intra-hepatic shunts were measured in vitro and the point of confluence between the hepatic artery and portal vein in the rat determined. Livers of male Sprague-Dawley rats were single-pass, dual-perfused in vitro. Total cessation or diversion of the hepatic arterial inflow to the portal venous vasculature, whilst maintaining total hepatic perfusate flow. decreased intrasinusoidal pressure, increased transhepatic portal venous resistance and opened the portal venous intrahepatic shunts in a manner similar to intraportal injection of 15-pm diameter microspheres. Injections of the microspheres into the hepatic arterial circulation increased hepatic arterial pressure dramatically, consistent with complete occlusion of the arterial vasculature. The intrahepatic shunts are located at a pre-sinusoidal level because no increases were detected in hepatic arterial pressure following intraportal injection of microspheres. The hepatic arterial vasculature, unlike the portal supply, does not possess a collateral shunt circulation and coalesces with the portal vein at an intrasinusoidal location.