Membrane Barrels Are Taller, Fatter, Inside-Out Soluble Barrels.
Membrane Barrels Are Taller, Fatter, Inside-Out Soluble Barrels.
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DOI：
10.1021/acs.jpcb.1c00878
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发表时间：
2021-04-15
期刊：
影响因子：
--
通讯作者：
Slusky JSG
中科院分区：
文献类型：
--
作者：
Dhar R;Feehan R;Slusky JSG
上下β-桶拓扑结构存在于膜和可溶性环境中。然而，β桶实际上是存在于外膜中的唯一拓扑结构。通过比较这些结构相似的蛋白质的特征，我们可以确定哪些特征是环境特有的，而不是折叠。在这里，我们比较了膜β-桶的结构，可溶性β-桶，并评估其相对大小，形状，氨基酸组成，疏水性和周期性。我们发现，膜β-桶通常比可溶性β-桶大，每桶有更多的链，每条链有更多的氨基酸，使它们更宽更高。我们还发现膜β-桶是由内而外的可溶性β-桶。膜β-桶的内部区域具有与可溶性β-桶的外部区域相似的疏水性，并且膜β-桶的外部区域具有与可溶性β-桶的内部区域相似的疏水性。此外，即使假设两种类型的β-桶都具有方向和疏水性交替的氨基酸链，我们发现膜β-桶比可溶性β-桶具有更有规律的交替。这些特征使我们深入了解膜桶如何在膜中保持其折叠和功能。
Up-and-down β-barrel topology exists in both the membrane and soluble environment. However, β-barrels are virtually the only topology that exist in the outer membrane. By comparing features of these structurally similar proteins, we can determine what features are particular to the environment rather than the fold. Here we compare structures of membrane β-barrels to soluble β-barrels and evaluate their relative size, shape, amino acid composition, hydrophobicity, and periodicity. We find that membrane β-barrels are generally larger than soluble β-barrels in with more strands per barrel and more amino acids per strand, making them wider and taller. We also find that membrane β-barrels are inside-out soluble β-barrels. The inward region of membrane β-barrels have similar hydrophobicity to the outward region of soluble β-barrels, and the outward region of membrane β-barrels has similar hydrophobicity to the inward region of the soluble β-barrels. Moreover, even though both types of β-barrel have been assumed to have strands with amino acids that alternate in direction and hydrophobicity, we find that the membrane β-barrels have more regular alternation than soluble β-barrels. These features give insight into how membrane barrels maintain their fold and function in the membrane.
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影响因子：
8
作者：
Hsieh, Daniel;Davis, Alexander;Nanda, Vikas
通讯作者：
Nanda, Vikas
影响因子：
3.4
作者：
Javadpour, MM;Eilers, M;Smith, SO
通讯作者：
Smith, SO
影响因子：
3.4
作者：
Eilers, M;Patel, AB;Smith, SO
通讯作者：
Smith, SO
DOI：
10.1073/pnas.97.11.5796
发表时间：
2000-05-23
影响因子：
11.1
作者：
Eilers, M;Shekar, SC;Fleming, PJ
通讯作者：
Fleming, PJ
影响因子：
3.4
作者：
Gimpelev, M;Forrest, LR;Honig, B
通讯作者：
Honig, B