Preparation of bioactive amide compounds from black pepper by countercurrent chromatography and preparative HPLC
Preparation of bioactive amide compounds from black pepper by countercurrent chromatography and preparative HPLC
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DOI：
10.1016/j.indcrop.2012.11.019
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发表时间：
2013-01-01
影响因子：
5.9
通讯作者：
Du, Qizhen
中科院分区：
文献类型：
--
作者：
Jin, Yongyang;Qian, Dengyong;Du, Qizhen
酰胺类化合物是黑胡椒中的主要生物活性物质。采用高速逆流色谱法对黑胡椒提取物进行分离，得到三种化合物：脱氢胡椒碱、胡椒碱和胡椒醛。（2 E，4 E，12 Z）-N-异丁基-十八碳三烯酰胺和四个级分，通过制备型HPLC分离，得到十种纯化的化合物：逆苯酰胺-A，（2 E，4 E）-N-异丁基-癸二烯酰胺，逆苯酰胺-C，胡椒油精A，脱氢胡椒油精A，逆苯酰胺-B，胡椒油精B，胡椒壬碱，哌啶环丁酰胺A和（2 E，4 E，14 Z）-N-异丁基二十碳-2，4，14-三烯酰胺。本研究结果表明，高速逆流色谱法是一种从黑胡椒中制备具有生物活性的酰胺类化合物的有效方法。(C)2012爱思唯尔有限公司版权所有。
Amide compounds are the major bioactive substances in black pepper. Black pepper extracts were fractionated by high-speed countercurrent chromatography resulting in three compounds: dehydropipernonaline, piperine and (2E,4E,12Z)-N-isobutyl-octadecatrienamide, and four fractions which were separated by preparative HPLC to yield ten purified compounds: retrofractamide-A, (2E,4E)-N-isobutyl-decadienamide, retrofractamide-C, piperoleine A, dehydropiperoleine A, retrofractamide-B, piperoleine B, pipernonaline, pipercyclobutanamides A and (2E,4E,14Z)-N-isobutyleicosa-2,4,14-trienamide as identified by ESI-MS and NMR analysis. The results of the present study indicated that HSCCC is an effective technique for the preparation of bioactive amide compounds from black pepper. (C) 2012 Elsevier B.V. All rights reserved.