MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae
MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae
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DOI：
10.1073/pnas.0606165104
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发表时间：
2007-05-22
影响因子：
11.1
通讯作者：
Dresser, Michael E.
中科院分区：
文献类型：
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作者：
Conrad, Michael N.;Lee, Chih-Ying;Dresser, Michael E.
在减数分裂前期，端粒与核膜相关联，并在中心体/纺锤体极附近积累以形成染色体束，这是酿酒酵母中非常保守的事件，需要减数分裂端粒蛋白Ndj 1 p。Ndj 1 p与Mps 3 p相互作用，Mps 3 p是一种核被膜SUN结构域蛋白，是纺锤体极体复制和姐妹染色单体凝聚所必需的。从MPS 3中去除Ndj 1 p相互作用结构域会产生一个类似ndj 1 Delta的功能分离等位基因，Ndj 1 p和Mps 3 p与端粒稳定相关。SUN结构域蛋白存在于跨门的核膜中，并且涉及介导细胞核内部与细胞骨架之间的相互作用。我们的观察表明了减数分裂端粒运动的一般机制。
In meiotic prophase, telomeres associate with the nuclear envelope and accumulate adjacent to the centrosome/spindle pole to form the chromosome bouquet, a well conserved event that in Saccharomyces cerevisiae requires the meiotic telomere protein Ndj1p. Ndj1p interacts with Mps3p, a nuclear envelope SUN domain protein that is required for spindle pole body duplication and for sister chromatid cohesion. Removal of the Ndj1p-interaction domain from MPS3 creates an ndj1 Delta-like separation-of-function allele, and Ndj1p and Mps3p are codependent for stable association with the telomeres. SUN domain proteins are found in the nuclear envelope across phyla and are implicated in mediating interactions between the interior of the nucleus and the cytoskeleton. Our observations indicate a general mechanism for meiotic telomere movements.