Muscle Differentiation and Myotubes Alignment Is Influenced by Micropatterned Surfaces and Exogenous Electrical Stimulation
Muscle Differentiation and Myotubes Alignment Is Influenced by Micropatterned Surfaces and Exogenous Electrical Stimulation
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DOI：
10.1089/ten.tea.2008.0301
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发表时间：
2009-09-01
影响因子：
4.1
通讯作者：
Elvassore, Nicola
中科院分区：
文献类型：
--
作者：
Flaibani, Marina;Boldrin, Luisa;Elvassore, Nicola
一个在体外的肌肉样结构与平行取向的收缩肌管是需要作为一个模型的肌肉组织再生。为此，有必要重现一个可控的微观环境模仿体内的线索。在这项工作中，我们专注于应用拓扑和电刺激的肌肉前体细胞（MPC）的培养，以影响MPC的方向和诱导肌管排列。这两种刺激都进行了测试，独立和一起。使用微图案化的聚-（L-乳酸）膜实现结构和拓扑模板。对MPC培养物施加由每30秒70 mV/cm振幅的方波脉冲组成的电刺激。通过趋电性分析评价不同脉冲持续时间对培养物的影响。最高的细胞位移率朝向阴极观察到3毫秒脉冲刺激，然后与拓扑刺激相结合。拓扑刺激和电刺激在增强培养的MPC的分化方面具有相加效应，表现为高肌钙蛋白I蛋白产生以及平行的肌细胞生成素和结蛋白基因分别下调和上调。
An in vitro muscle-like structure with parallel-oriented contractile myotubes is needed as a model of muscle tissue regeneration. For this purpose, it is necessary to reproduce a controllable microscale environment mimicking the in vivo cues. In this work we focused on the application of topological and electrical stimuli on muscle precursor cell (MPC) culture to influence MPC orientation and induce myotube alignment. The two stimulations were tested both independently and together. A structural and topological template was achieved using micropatterned poly-(L-lactic acid) membranes. Electrical stimulation, consisting of square pulses of 70mV/cm amplitude each 30 s, was applied to the MPC culture. The effect of different pulse durations on cultures was evaluated by galvanotaxis analysis. The highest cell displacement rate toward the cathode was observed for 3 ms pulse stimulation, which was then applied in combination with topological stimuli. Topological and electrical stimuli had an additive effect in enhancing differentiation of cultured MPC, shown by high Troponin I protein production and, in parallel, Myogenin and Desmin genes, down-and upregulation respectively.