Aerobic secondary utilization of a non-growth and inhibitory substrate 2,4,6-trichlorophenol by Sphingopyxis chilensis S37 and Sphingopyxis-like strain S32
Aerobic secondary utilization of a non-growth and inhibitory substrate 2,4,6-trichlorophenol by Sphingopyxis chilensis S37 and Sphingopyxis-like strain S32
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DOI：
10.1023/a:1024752605059
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发表时间：
2003-08-01
期刊：
影响因子：
3.6
通讯作者：
Martínez, M
中科院分区：
文献类型：
--
作者：
Aranda, C;Godoy, F;Martínez, M
本文报道了不能以246TCP作为唯一碳源和能量源的Sphingopyxis chilensis S37和Sphingopyxis chilensis样菌株S32对2,4,6-三氯苯酚（246TCP）的降解。在R2A培养液中，菌株对246TCP的降解深度可达0.5 mM。结果表明，在不同246TCP和葡萄糖浓度的无矿盐培养基中，细菌对葡萄糖的依赖使246TCP能够生长和降解。同时添加5.33 mM葡萄糖时，菌株S32降解盐酚的深度为0.2 mM；同时添加1.33 mM葡萄糖时，菌株S37降解盐酚的深度为0.1 mM。在没有葡萄糖的情况下，这些246TCP浓度对接种者是致命的。氯离子的化学计量释放量以及HPLC、GC-ECD和GC-MS分析表明，两株菌株均发生了246TCP矿化。据我们所知，这是第一次报道细菌能够矿化氯酚作为非生长和抑制底物。针对该活性，提出了二次利用而非共代谢的概念。
This paper reports 2,4,6-trichlorophenol (246TCP) degradation by Sphingopyxis chilensis S37 and Sphingopyxis chilensis-like strain S32, which were unable to use 246TCP as the sole carbon and energy source. In R2A broth, the strains degraded 246TCP up to 0.5 mM. Results with mixtures of different 246TCP and glucose concentrations in mineral salt media demonstrated dependence on glucose to allow bacterial growth and degradation of 246TCP. Strain S32 degraded halophenol up to 0.2 mM when 5.33 mM glucose was simultaneously added, while strain S37 degraded the compound up to 0.1 mM when 1.33 mM glucose was added. These 246TCP concentrations were lethal for inocula in absence of glucose. Stoichiometric releases of chloride and analysis by HPLC, GC-ECD and GC-MS indicated 246TCP mineralisation by both strains. To our knowledge, this is the first report of bacteria able to mineralize a chlorophenol as a non-growth and inhibitory substrate. The concept of secondary utilization instead of cometabolism is proposed for this activity.