Motility of Enzyme-Powered Vesicles
Motility of Enzyme-Powered Vesicles
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DOI：
10.1101/645986
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发表时间：
2019-05
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影响因子：
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通讯作者：
Subhadip Ghosh;Farzad Mohajerani;S. Son;D. Velegol;P. Butler;Ayusman Sen
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文献类型：
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作者：
Subhadip Ghosh;Farzad Mohajerani;S. Son;D. Velegol;P. Butler;Ayusman Sen
由化学催化产生的能量驱动的自主纳米车辆具有作为活性递送剂的潜在应用。对于体内应用，发动机及其燃料以及底盘本身必须是生物相容的。酶分子已被证明通过底物周转产生机械力，并且是作为发动机的有吸引力的候选者;磷脂囊泡是生物相容的，并且可以用作货物容器。在这里，我们描述了自主运动的囊泡与膜结合酶的存在下的基板。我们发现，囊泡的运动性随着酶周转率的增加而增加。通过使用光学显微镜跟踪囊泡的运动，进一步证实了这些酶动力系统的增强扩散在真实的时间。通过将化学能转换成机械运动而移动的膜结合的原始细胞充当能动活细胞的模型，并且是阐明支配活性膜动力学和细胞运动的基本机制的关键。“仅适用于目录”
Autonomous nanovehicles powered by energy derived from chemical catalysis have potential applications as active delivery agents. For in vivo applications, it is necessary that the engine and its fuel, as well as the chassis itself, be biocompatible. Enzyme molecules have been shown to generate mechanical force through substrate turnover and are attractive candidates as engines; phospholipid vesicles are biocompatible and can serve as cargo containers. Herein, we describe the autonomous movement of vesicles with membrane-bound enzymes in the presence of the substrate. We find that the motility of the vesicles increases with increasing enzymatic turnover rate. The enhanced diffusion of these enzyme-powered systems was further substantiated in real time by tracking the motion of the vesicles using optical microscopy. The membrane-bound protocells that move by transducing chemical energy into mechanical motion serve as models for motile living cells and are key to the elucidation of the fundamental mechanisms governing active membrane dynamics and cellular movement. “For Table of Contents Only”