Twofold polyketide branching by a stereoselective enzymatic Michael addition
Twofold polyketide branching by a stereoselective enzymatic Michael addition
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DOI：
10.1039/c5cc03085d
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Hertweck, Christian
中科院分区：
文献类型：
--
作者：
Heine, Daniel;Sundaram, Srividhya;Hertweck, Christian
使用聚酮化合物模拟物和支链（二）甲基丙二酸单酰辅酶A延伸单元，在体外酶测定中测试了根茎素聚酮化合物合酶的分支模块的多功能性。产品与合成参考化合物的比较表明，该模块能够通过迈克尔加成-内酯化序列一步立体选择性地引入两个分支，从而扩展了先前研究的 PKS 系统的范围。
The versatility of the branching module of the rhizoxin polyketide synthase was tested in an in vitro enzyme assay with a polyketide mimic and branched (di) methylmalonyl-CoA extender units. Comparison of the products with synthetic reference compounds revealed that the module is able to stereoselectively introduce two branches in one step by a Michael addition-lactonisation sequence, thus expanding the scope of previously studied PKS systems.