Distribution of schistosome genetic diversity within naturally infected Rattus rattus detected by RAPD markers
Distribution of schistosome genetic diversity within naturally infected Rattus rattus detected by RAPD markers
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DOI：
10.1017/s003118200006755x
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发表时间：
1996-12-01
期刊：
影响因子：
2.4
通讯作者：
Theron, A
中科院分区：
文献类型：
--
作者：
Barral, V;Morand, S;Theron, A
采用随机扩增多态性DNA标记（RAPD）技术，对在瓜德罗普岛捕获的曼氏血吸虫（Schistosoma mansoni）自然宿主鼠（Rattus Rattus）种群内和种群间的遗传多样性进行了可视化分析。表型由寄生物的性别和3个引物产生的8个多态性标记表征。在10只感染大鼠的212个寄生虫个体中，鉴定出78个基因型。所有自然感染的宿主均携带多种基因型，每种宿主最多可携带28种基因型。由Shannon-Wiener指数和Lynch和Milligan估计分别计算的表型和基因型多样性在宿主内部平均大于宿主之间。考虑到本研究中观察到的极低的蜗牛感染率和脊椎动物宿主的快速更替，我们的研究结果表明，脊椎动物宿主的高流动性和/或多酸蜗牛感染可能是导致宿主内寄生虫遗传多样性的因素。
Random amplified polymorphic DNA markers (RAPD) were used to visualize the genetic diversity within and between infrapopulations of Schistosoma mansoni recovered from the natural vertebrate host, Rattus rattus, trapped at an insular Guadeloupean focus. Phenotypes were characterized by the sex of the parasites and by 8 polymorphic markers generated by 3 primers. Among the 212 parasite individuals recovered from 10 infected rats, 78 genotypes were characterized. All the hosts naturally infected harboured multiple parasite genotypes with a maximum diversity of 28 genotypes/host. Phenotypic and genotypic diversity calculated by Shannon-Wiener's indices and Lynch and Milligan's estimators respectively is, on average, greater within than between hosts. Considering the very low snail infection rates observed in this focus and the rapid turnover of the vertebrate hosts, our results suggest that the high mobility of the vertebrate host and/or plurimiracidial snail infections could be factors responsible for parasite genetic diversity within hosts.