Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues
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DOI：
10.1099/vir.0.82757-0
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发表时间：
2007-08-01
影响因子：
3.8
通讯作者：
Grimes, Jonathan M.
中科院分区：
文献类型：
--
作者：
Assenberg, Rene;Ren, Jingshan;Grimes, Jonathan M.
我们已经确定了高分辨率的晶体结构的甲基转移酶结构域的NS 5多肽从墨累谷脑炎病毒。该结构域在具有Cap 1合成所需的N7和2 '-O甲基转移酶活性方面是不寻常的。我们还确定了该结构域的复合物的结构与核苷和帽类似物提供的信息帽结合，基于此，我们建议一个模型的顺序甲基化的N7和2 '-O组的帽可能是协调的。
We have determined the high resolution crystal structure of the m ethylt ransfe rase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleoticles and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.