Maternal N-Carbamylglutamate Supply during Early Pregnancy Enhanced Pregnancy Outcomes in Sows through Modulations of Targeted Genes and Metabolism Pathways
Maternal N-Carbamylglutamate Supply during Early Pregnancy Enhanced Pregnancy Outcomes in Sows through Modulations of Targeted Genes and Metabolism Pathways
复制标题
妊娠早期母体 N-氨甲酰谷氨酸供应通过调节目标基因和代谢途径增强母猪妊娠结局
DOI：
10.1021/acs.jafc.8b01637
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发表时间：
2018-06-13
影响因子：
6.1
通讯作者：
Zeng, Xiangfang
中科院分区：
文献类型：
--
作者：
Cai, Shuang;Zhu, Jinlong;Zeng, Xiangfang
减少妊娠损失对于提高人类和哺乳动物的生殖效率都很重要。我们以前的研究表明，母体N-氨甲酰谷氨酸（NCG）供应在妊娠早期提高母猪胚胎存活。然而，产妇NCG供应是否改善妊娠结局仍不清楚。本研究发现，母猪妊娠早期补充NCG可显著提高每窝活产仔猪数（P < 0.05），并显著改变羊水和血清中参与能量、脂质、谷胱甘肽代谢和免疫调节的代谢物水平。补充NCG可显著增加子宫内膜孕酮受体膜成分1（PGRMC 1）的表达（P < 0.05），并增加PGRMCI、内皮型一氧化氮合酶（eNOS）和核纤层蛋白A/C的表达（P < 0.05）。在NCG相关氨基酸中，精氨酸和谷氨酰胺能显著增加猪滋养外胚层细胞PGRMC 1和eNOS的表达（P < 0.05），谷氨酸能刺激猪滋养外胚层细胞vimentin和laminA/C的表达（P < 0.05），脯氨酸能刺激laminA/C的表达（P < 0.05）。总的来说，这些数据揭示了NCG减少早期胚胎丢失的机制。这些发现具有重要意义，NCG具有改善人类和哺乳动物妊娠结局的巨大潜力。
Reducing pregnancy loss is important for improving reproductive efficiency for both human and mammalian animals. Our previous study demonstrates that maternal N-carbamylglutamate (NCG) supply during early pregnancy enhances embryonic survival in gilts. However, whether maternal NCG supply improves the pregnancy outcomes is still not known. Here we found maternal NCG supply during early pregnancy in sows significantly increased the numbers of total piglets born alive per litter (P < 0.05) and significantly changed the levels of metabolites in amniotic fluid and serum involved in metabolism of energy, lipid, and glutathione and immunological regulation. The expression of endometrial progesterone receptor membrane component 1 (PGRMC1) was significantly increased by NCG supplementation (P < 0.05) as well as the expression of PGRMCI, endothelial nitric oxide synthesases (eNOS), and lamin A/C in fetuses and placentae (P < 0.05). Among the NCGassociated amino acids, arginine and glutamine, markedly increased PGRMC1 and eNOS expression in porcine trophectoderm cells (P < 0.05), whereas glutamate could stimulate the expression of vimentin and lamin A/C in porcine trophectoderm (pTr) cells (P < 0.05) and proline stimulated lamin A/C expression (P < 0.05). Collectively, these data reveal the mechanisms of NCG in reducing early embryo loss. These findings have important implications that NCG has great potential to improve pregnancy outcomes in human and mammalian animals.