The effect of chlortetracycline on faecal microbial populations in growing swine
The effect of chlortetracycline on faecal microbial populations in growing swine
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DOI：
10.1016/j.jgar.2013.04.004
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发表时间：
2013-09-01
影响因子：
4.6
通讯作者：
Nisbet, D. J.
中科院分区：
文献类型：
--
作者：
Poole, T. L.;Suchodolski, J. S.;Nisbet, D. J.
随着细菌对多种抗菌剂耐药性的积累，抗菌剂的使用对食用动物胃肠道微生物区系的影响越来越受到关注。只有一小部分胃肠道微生物群是可培养的，这使得猪胃肠道生态系统的特征变得复杂。本研究的目的是用一种不依赖培养的方法来确定生长促进剂金霉素(50g/吨)对猪粪中细菌系统发育多样性的影响。4头新断奶仔猪饲喂以玉米(63.7%)和豆粕(25.2%)为主的饲粮，饲喂21d；21日龄饲粮中添加50g/吨金霉素，连续饲喂4周。在第0、14、23、28、35、42和49天从每头猪身上采集粪便，进行16S rRNA基因的454焦磷酸测序。焦磷酸测序数据的UniFrac分析表明，基于日粮和饲喂低剂量金霉素的猪(P>；0.05)之间的细菌多样性没有显著差异。两个处理组中最丰富的门是菲尔米特菌、类杆菌、变形杆菌和螺旋藻。可能需要较高浓度的金霉素(例如200克/吨或400克/吨)才能观察到猪粪便中的胃肠道菌群与本研究中的低剂量相比发生了变化。需要进行更广泛的研究，以彻底了解促进生长的抗菌剂如何影响肠道微生物区系，并有利于食用动物的生长效率。爱思唯尔有限公司代表国际感染和癌症化疗学会出版。
The effect of antimicrobial use on the gastrointestinal microbiota of food animals is of increasing concern as bacteria accumulate resistance to multiple antimicrobials. Only a small fraction of the gastrointestinal microbiome is culturable, complicating characterisation of the swine gastrointestinal ecosystem. The objective of this study was to determine the effect of a growth promotion dose (50 g/ton) of chlortetracycline on the phylogenetic diversity of bacteria from swine faeces using a culture-independent method. Four freshly weaned pigs were provided a grower ration of primarily corn (63.7%) and soybean meal (25.2%) for 21 days; on Day 21 for 4 weeks the diet of two pigs was medicated with 50 g/ton chlortetracycline. Faecal material was collected from each pig on Days 0, 14, 23, 28, 35, 42 and 49 for 454-pyrosequencing of the 16S rRNA gene. UniFrac analysis of pyrosequencing data showed no significant difference in bacterial diversity based on diet and among pigs (P>0.05) fed the low-level dose of chlortetracycline. The most abundant phyla in both treatment groups were Firmicutes, Bacteroidetes, Proteobacteria and Spirochaetes. Higher concentrations of chlortetracycline (e.g. 200 g/ton or 400 g/ton) may be required to observe a shift in the gastrointestinal flora in swine faeces compared with the low-level dose in this study. Studies of broader scope are needed to understand thoroughly how growth-promoting antimicrobials influence the gut microflora and benefit food animal growth efficiency. Published by Elsevier Ltd on behalf of International Society for Chemotherapy of Infection and Cancer.