Testosterone induces Ca2+ influx via non-genomic surface receptors in activated T cells
Testosterone induces Ca2+ influx via non-genomic surface receptors in activated T cells
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DOI：
10.1016/s0014-5793(97)00346-3
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发表时间：
1997-04-28
期刊：
影响因子：
3.5
通讯作者：
Wunderlich, F
中科院分区：
文献类型：
--
作者：
Benten, WPM;Lieberherr, M;Wunderlich, F
用Fura-2方法研究了睾酮对C57BL/10雌性小鼠脾T细胞胞浆游离钙离子的直接作用。生理浓度为1-10 nM的睾酮在数秒内引起[Ca~(2+)](I)升高，这是由于Ca~(2+)内流，而不是由细胞内钙释放引起的。相反，雌二醇可引起Ca~(2+)内流和Ca~(2+)释放。睾酮诱导的Ca~(2+)内流是由Ni2+受体阻滞剂介导的，不受经典雄激素受体阻滞剂环丙孕酮的抑制。这些数据表明，睾酮对T细胞的直接作用是一种新的方式，它不是通过经典的雄激素受体反应而是通过非传统的质膜受体介导的。(C)1997年欧洲生化学会联合会。
Using the Fura-2 method we investigated a possible direct action of testosterone on cytosolic free calcium of splenic T cells isolated from female C57BL/10 mice, Testosterone at physiological concentrations of 1-10 nM induces an increase in [Ca2+](i) within seconds, which is due to Ca2+ influx and not to Ca2+ release from intracellular stores, In contrast, estradiol induces both Ca2+ influx and Ca2+ release, The testosterone-induced Ca2+ influx is mediated by Ni2+-blockable channels and is not inhibited by cyproterone, a blocker of the classical androgen receptor, Ca2+ influx can also be induced by testosterone conjugated to BSA which is impermeable to the plasma membrane, These data indicate a novel mode of direct action of testosterone on T cells which is not mediated through the classical androgen receptor response, but through unconventional plasma membrane receptors. (C) 1997 Federation of European Biochemical Societies.