PROSTATIC TUMOR (R3327) SKELETAL METASTASIS
PROSTATIC TUMOR (R3327) SKELETAL METASTASIS
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DOI：
10.1002/pros.2990160402
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发表时间：
1990-01-01
期刊：
影响因子：
2.8
通讯作者：
RAO, BR
中科院分区：
文献类型：
--
作者：
GELDOF, AA;RAO, BR
在下腔静脉同时暂时闭塞的情况下，通过尾静脉向雄性哥本哈根大鼠接种单分散的R3327-MatLyLu前列腺肿瘤细胞，以建立前列腺癌骨转移的动物模型。该手术可重复地导致脊柱腰椎区域的转移性肿瘤生长。在显微镜下，接种后4天内，在第五和第六腰椎中可见肿瘤生长。术后14天内出现神经功能丧失的临床体征（后肢轻瘫和麻痹）。细胞培养技术证实了在第五和第六腰椎的空间管内存在存活的、增殖的肿瘤细胞群。在组织学上，在腰椎中观察到明显的骨细胞反应和伴随的成骨细胞活性。在血清中，可以证明一过性高钙血症。在这些动物中骨转移的发展并没有反映在血清酸性磷酸酶，骨特异性抗原，或骨钙素水平的显着变化。这些观察结果支持了脊椎静脉丛参与前列腺肿瘤细胞播散的概念。所描述的外科手术允许前列腺癌骨转移的实验研究。
Male Copenhagen rats were inoculated with monodispersed R3327-MatLyLu prostate tumor cells via the tail vein under concomittant temporal occlusion of the inferior vena cava to develop an animal model for skeletal metastasis of prostate cancer. This procedure reproducibly resulted in metastatic tumor growth in the lumbar region of the vertebral column. Microscopically, tumor growth became visible in the fifth and sixth lumbar vertebrae within 4 days after inoculation. Clinical signs of nerve function disablement (hind leg paresis and paralysis) followed within 14 days of such a procedure. Cell culture technique confirmed the presence of a viable, proliferating tumor cell population within the spatial canal of the fifth and sixth lumbar vertebrae. Histologically, a clear response of osteoclastic and concomittant osteoblastic activities was observed in the lumbar spinal column. In the serum, a transient phase of hypercalcemia could be demonstrated. The development of skeletal metastases in these animals was not reflected by significant alteration in serum levels of acid phosphatase, prostatic-specific antigen, or osteocalcin. These observations support the concept of the vertebral venous plexus being involved in the dissemination of prostate tumor cells. The surgical procedures described permit experimental investigations of bone metastasis of prostatic cancer.