Measurement of immunoglobulin M, immunoglobulin G, and immunoglobulin A antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: comparison of lipopolysaccharide and whole bacterium as antigen
Measurement of immunoglobulin M, immunoglobulin G, and immunoglobulin A antibodies against Yersinia enterocolitica by enzyme-linked immunosorbent assay: comparison of lipopolysaccharide and whole bacterium as antigen
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通过酶联免疫吸附试验测定抗小肠结肠炎耶尔森菌的免疫球蛋白 M、免疫球蛋白 G 和免疫球蛋白 A 抗体：脂多糖和全细菌作为抗原的比较
DOI：
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发表时间：
1981
影响因子：
9.4
通讯作者：
A. Toivanen
中科院分区：
文献类型：
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作者：
K. Granfors;M. Viljanen;A. Toivanen
本发明描述了一种酶联免疫吸附试验（ELISA），其用于通过使用脂多糖作为抗原来检测和定量针对小肠结肠炎耶尔森氏菌的人免疫球蛋白M（IgM）、IgG和伊加抗体。将脂多糖ELISA法与全菌ELISA法的结果进行了比较。对于每种免疫球蛋白类别，观察到的相关性良好。Y.小肠结肠炎血清型O：3和O：9，假结核耶尔森氏菌IA，和流产布鲁氏菌进行了研究，在整个细菌和脂多糖ELISA的人和兔抗血清和兔抗血清使用ELISA抑制。观察到的最大交叉反应性是抗布鲁氏菌血清与Y。小肠结肠炎菌O：9全菌ELISA。在脂多糖ELISA中，这种交叉反应与兔抗血清不明显，但与人抗血清的交叉反应很强。然而，ELISA抑制的鉴别诊断是可能的。根据我们的经验，我们现在常规使用全菌ELISA来测定类别特异性耶尔森氏菌抗体，并且通过ELISA抑制来控制潜在的交叉反应。
An enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by using lipopolysaccharides as antigens is described. The results obtained with the lipopolysaccharide ELISA were compared with the results of the whole bacterium ELISA. The correlations observed were good for each immunoglobulin class. Cross-reactions between Y. enterocolitica serotypes O:3 and O:9, Yersinia pseudotuberculosis IA, and Brucella abortus were studied by human and rabbit antisera in the whole bacterium and lipopolysaccharide ELISAs and by rabbit antisera using ELISA inhibition. The greatest cross-reactivity observed was that of the anti-Brucella serum with Y. enterocolitica O:9 in the whole bacterium ELISA. In the lipopolysaccharide ELISA this cross-reaction was not demonstrable with the rabbit antiserum, but it was strong with the human antiserum. However, differential diagnosis was possible with ELISA inhibition. On the basis of our experience, we are now routinely using whole bacterium ELISA for the determination of class-specific Yersinia antibodies, and potential cross-reactions are controlled by the ELISA inhibition.