CXCR4pos circulating progenitor cells coexpressing monocytic and endothelial markers correlating with fibrotic clinical features are present in the peripheral blood of patients affected by systemic sclerosis
CXCR4pos circulating progenitor cells coexpressing monocytic and endothelial markers correlating with fibrotic clinical features are present in the peripheral blood of patients affected by systemic sclerosis
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DOI：
10.3324/haematol.12526
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发表时间：
2008-08-01
期刊：
影响因子：
--
通讯作者：
Trotta, Francesco
中科院分区：
文献类型：
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作者：
Campioni, Diana;Lo Monaco, Andrea;Trotta, Francesco
关于循环内皮细胞和祖细胞（CEC/CEP）在系统性硬化症（SSc）发病机制中的作用仍存在争议。使用基于四色流式细胞术方案的顺序布尔门控策略，与健康受试者相比，在SSc患者中检测到CD 31（pos）/CD 184（pos）（CXCR 4）/CD 34（pos）/CD 45（pos）和CD 31（pos）/CD 117（pos）（c-kit-R）/CD 34（pos）/CD 45（pos）造血循环祖细胞（HCPC）数量增加。在SSc中，没有观察到循环中的成熟内皮细胞和祖细胞，而红系祖细胞的生成增强被发现与CD 117 + HCPC的存在相关。新鲜检测到的CXCR 4posHCPC的存在与体外培养的梭形内皮样细胞（SELC）（具有内/骨髓单核细胞特征）或SDF-1和VEGF血清水平相关。这些数据与该疾病的更多纤维化临床特征相关，从而支持这些细胞在纤维化中的可能作用。
There is still controversy regarding the role of circulating endothelial and progenitor cells (CECs/CEPs) in the pathogenesis of systemic sclerosis (SSc). Using a sequential Boolean gating strategy based on a 4-color flow cytometric protocol, an increased number of CD31(pos)/CD184(pos)(CXCR4)/CD34(pos)/CD45(pos) and CD31(pos)/CD117(pos) (c-kit-R) /CD34(pos)/ CD45(pos) hematopoietic circulating progenitor cells (HCPCs) was detected in SSc patients compared with healthy subjects. In SSc, no circulating mature and progenitor endothelial cells were observed, while an enhanced generation of erythroid progenitor cells was found to be correlated with the presence of CD117+ HCPCs. The presence of freshly detected CXCR4posHCPC was correlated either to the in vitro cultured spindle-shaped endothelial like cells (SELC) with an endo/myelomonocytic profile or to SDF-1 and VEGF serum level. These data are related to more fibrotic clinical features of the disease, thus supporting a possible role of these cells in fibrosis.