Plasma iron and transferrin iron-binding capacity evaluated by colorimetric and immunoprecipitation methods.
Plasma iron and transferrin iron-binding capacity evaluated by colorimetric and immunoprecipitation methods.
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DOI：
10.1093/clinchem/33.2.273
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发表时间：
1987-02
影响因子：
9.3
通讯作者：
H. Huebers;M. Eng;B. Josephson;N. Ekpoom;R. Rettmer;R. Labbé;P. Pootrakul;C. Finch
中科院分区：
文献类型：
--
作者：
H. Huebers;M. Eng;B. Josephson;N. Ekpoom;R. Rettmer;R. Labbé;P. Pootrakul;C. Finch
我们评估了正常个体和铁失衡患者的血浆铁（PI）和总铁结合能力（TIBC）或转铁蛋白。将PI和TIBC的标准比色法和TIBC的标准同位素稀释法与免疫沉淀法和转铁蛋白免疫电泳法进行了比较。标准法和免疫沉淀法测量的PI浓度对所有个体都非常一致，除了那些含有饱和转铁蛋白的个体，其中非转铁蛋白铁增加了标准测定的结果。饱和血浆中多余的铁可能来自游离铁或含铁铁蛋白。两种方法的TIBC也存在差异。免疫电泳检测铁缺乏血清中转铁蛋白的含量较高。同位素稀释法在某些铁饱和血浆中增加了不饱和铁的结合能力，在计算TIBC时加上错误的高PI值会产生复合误差。在同位素稀释法中，加入的铁和转铁蛋白之间可能发生了一些铁交换。这些测量结果证实了除铁饱和血浆外，测量PI和TIBC的标准比色法的准确性。然而，测量PI和TIBC的多克隆免疫沉淀方法的更大特异性使得它在区分转铁蛋白结合铁和非转铁蛋白结合铁方面特别有用。
We evaluated plasma iron (PI) and total iron-binding capacity (TIBC) or transferrin in normal individuals and in patients with iron imbalance. The standard colorimetric measurements of PI and TIBC and the standard isotope-dilution measurement of TIBC were compared with an immunoprecipitation method and also with immunoelectrophoresis of transferrin. PI concentrations as measured by the standard and immunoprecipitation methods agreed closely for all individuals except those with saturated transferrin, where nontransferrin iron increased the results in the standard assay. This excess iron in saturated plasma may be derived from either free iron or iron-bearing ferritin. There were also differences in TIBC between the two methods. Iron-deficient sera gave higher values for transferrin when measured by immunoelectrophoresis. Unsaturated iron-binding capacity was increased in the isotope-dilution method in some iron-saturated plasma, compounding errors when added to erroneously high PI values to compute TIBC. Perhaps some exchange of iron occurred between added iron and transferrin iron in the isotope-dilution method. These measurements confirm the accuracy of the standard colorimetric method of measuring PI and TIBC except in iron-saturated plasma. However, the greater specificity of a polyclonal immunoprecipitation method of measuring PI and TIBC makes it particularly useful in differentiating transferrin-bound iron from nontransferrin iron.