Two divergent plasma membrane syntaxin-like SNAREs, nsyn1 and nsyn2, contribute to hyphal tip growth and other developmental processes in Neurospora crassa
Two divergent plasma membrane syntaxin-like SNAREs, nsyn1 and nsyn2, contribute to hyphal tip growth and other developmental processes in Neurospora crassa
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DOI：
10.1016/s1087-1845(03)00109-9
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发表时间：
2003-12-01
影响因子：
3
通讯作者：
Heath, IB
中科院分区：
文献类型：
--
作者：
Gupta, GD;Free, SJ;Heath, IB
菌丝顶端高度极化的胞吐作用是菌丝生长的必要条件。该要求可以通过基于顶端SNARE的机器的定位和/或激活来满足。我们已经克隆了nsyn 1和nsyn 2，预测在粗糙脉孢菌质膜上起作用的SNARE。将额外拷贝的nsyn 1转化为野生型菌株显示出与nsyn 1表达的抑制一致的效果，这在大多数转化体中是致命的。所有存活的转化子生长缓慢，分生孢子差，雄性不育。此外，反义nsyn 1菌株生长缓慢，菌丝直径和极性异常，分生孢子缺陷。对于nsyn 2，几个重复诱导的点突变（RIP）杂交产生没有或发芽不良的子囊孢子。那些发芽产生缓慢生长的菌丝与异常分支。nsyn 1和nsyn 2突变体中的缺陷与菌丝尖端和其他发育阶段的差异受损囊泡融合一致。(C)2003年爱思唯尔公司All rights reserved.
Highly polarized exocytosis of vesicles at hyphal apices is an essential requirement of tip growth. This requirement may be met by the localization and/or activation of an apical SNARE-based machinery. We have cloned nsyn1 and nsyn2, SNAREs predicted to function at the plasma membrane in Neurospora crassa. Transformation of extra copies of nsyn1 into wild-type strains displayed effects consistent with quelling of nsyn1 expression, which was lethal in most transformants. All surviving transformants grew slowly, conidiated poorly, and were male sterile. In addition, antisense nsyn1 strains grew slowly, with abnormal hyphal diameters and polarity and defective conidiation. For nsyn2, several repeat induced point mutation (RIP) crosses produced no, or poorly germinating ascospores. Those that germinated produced slow-growing hyphae with abnormal branching. The defects in nsyn1 and nsyn2 mutants are consistent with differential impaired vesicle fusion in hyphal tips and other developmental stages. (C) 2003 Elsevier Inc. All rights reserved.