Genomic analysis of synaptotagmin genes
Genomic analysis of synaptotagmin genes
复制标题
DOI：
10.1006/geno.2001.6619
复制
发表时间：
2001-09-01
期刊：
影响因子：
4.4
通讯作者：
Craxton, M
中科院分区：
文献类型：
--
作者：
Craxton, M
我使用TBLASTn探测DNA序列数据库，其中共有肽序列对应于啮齿动物突触结合蛋白（Syt）基因家族中最高度保守的区域，该区域位于C2B结构域内。我找到了所有已知啮齿动物基因的人类同源物，并发现了六个编码潜在家族成员的人类基因组位点。我在秀丽隐杆线虫中发现了8个潜在的家族成员，在黑腹果蝇中发现了6个，在拟南芥中发现了4个。梭线虫Syt1同源物唯一地编码两个可选择的C2B外显子，其中一个或另一个同时表达。Syt基因的基因组结构的比较清楚地表明了不同亚组的不同遗传。对基因组结构的了解将有助于系统地研究Syt基因中的选择性剪接。
I used TBLASTn to probe DNA sequence databases with a consensus peptide sequence corresponding to the most highly conserved region of the rodent synaptotagmin (Syt) gene family, which is within the C2B domain. I found human homologues for all known rodent genes, and found six further human genomic loci which encode potential family members. I found eight potential family members in Caenorhabditis elegans, six in Drosophila melanogaster, and four in Arabidopsis thaliana. The C. elegans Syt1 homologue uniquely encodes two alternative C2B exons, one or the other of which is expressed at a time. Comparison of the genomic structures of the Syt genes makes clear the different phylogenies of the different subgroups. Knowledge of the genomic structures will aid the systematic investigation of alternative splicing in Syt genes.