Expression of estrus modifies the gene expression profile in reproductive tissues on Day 19 of gestation in beef cows
Expression of estrus modifies the gene expression profile in reproductive tissues on Day 19 of gestation in beef cows
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DOI：
10.1016/j.theriogenology.2015.10.002
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发表时间：
2016-03-01
期刊：
影响因子：
2.8
通讯作者：
Cerri, R. L. A.
中科院分区：
文献类型：
--
作者：
Davoodi, S.;Cooke, R. F.;Cerri, R. L. A.
本研究旨在探讨人工授精（AI）发情对肉牛子宫内膜、妊娠和CL基因表达的影响。采用基于雌二醇和黄体酮（P4）的定时人工授精方案（AI =第0天），选取36头经产的非泌乳Nelore奶牛，并于第19天屠宰，采集子宫内膜、子宫内膜和妊娠。这些动物是根据(1)在Al附近有发情迹象（n = 19，发情）和(2)没有任何发情迹象（n = 17，未发情）的奶牛进行回顾性分组的。于实验第0、7、18天进行身体状况评分、采血、超声检查，提取58个靶基因的信使RNA并进行定量逆转录聚合酶链反应分析。使用程序GLM、MIXED和SAS的UNIVARIATE对重复测量的数据进行正态性检查和方差分析。只有怀孕的奶牛被纳入分析（n = 12；未发情的奶牛，n = 11）。情色表达与机体状况评分、排卵前卵泡直径、卵泡直径、Al后第7、18天血浆P4浓度、子宫冲红干扰素-tau浓度等参数无相关性（P < 0.05）；然而，发情奶牛的受胎尺寸显著增加（P = 0.02; 38.3 +/- 2.8 vs. 28.2 +/- 2.9 mm）。受子宫内膜发情表达影响的转录本大部分属于免疫系统和粘附分子家族(MX1、MX2、MYL12A、MMP19、CXCL10、IGLU和SLPI
The aim of this study was to test the effect of expression of estrus at artificial insemination (AI) on endometrium, conceptus, and CL gene expression of beef cows. Thirty-six multiparous nonlactating Nelore cows were enrolled on an estradiol-and progesterone (P4)-based timed AI protocol (AI = Day 0) and then slaughtered for the endometrium, CL, and conceptus collection on Day 19. The animals were retrospectively grouped on the basis of cows that (1) showed signs of estrus near Al (n = 19; estrus) and (2) did not show any signs ofestrus (n = 17; nonestrus). Body condition score, blood sampling, and ultrasound examination were performed on Days 0, 7, and 18 of the experiment followed by messenger RNA extraction and quantitative reverse transcription polymerase chain reaction analysis of 58 target genes. Data were checked for normality and analyzed by ANOVA for repeated measures using proc GLM, MIXED, and UNIVARIATE of SAS. Only pregnant cows were included in the analyses (n = 12; nonestrus, n = 11). Estrous expression had no correlation with parameters such as body condition score, preovulatory follicle and CL diameter, P4 concentration in plasma on Days 7 and 18 after Al, and interferon-tau concentration in the uterine flushing (P > 0.15); however, a significant increase was observed in conceptus size from cows that expressed estrus (P = 0.02; 38.3 +/- 2.8 vs. 28.2 +/- 2.9 mm). The majority of transcripts affected by estrous expression in the endometrium belong to the immune system and adhesion molecule family (MX1, MX2, MYL12A, MMP19, CXCL10, IGLU, and SLPI; P